System for preparing solutions for chromatography
A system for preparing solutions for chromatography application is disclosed. The system comprises a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution. The T-joint receives the second solution from a solution supply unit connected to the T-joint. Further one or more low pressure pumps supply the one or more first solutions into the T-joint. The high pressure pump collects the buffer solution and delivers it to a chromatography apparatus.
1. A system for performing inline blending of liquids, the system comprising:
a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution, wherein the T-joint receives the second solution from a solution supply unit connected to the T-joint, and the T-joint is connected to the solution supply unit using at least one direct connection tube, and wherein the T-joint is connected through at least one valve to a further solution supply unit for supplying the at least one first solution;
at least one low pressure pump for supplying the at least one first solution into the T-joint; and
a high pressure pump for collecting the buffer solution and delivering to a chromatography apparatus,
the system being characterized in that said T-joint includes a configuration for mixing the at least one first solution and the second solution, wherein the configuration for mixing the at least one first solution and the second solution is a magnetic stirrer,
wherein the system is configured such that the low-pressure pump, the T-joint, and the magnetic stirrer operate at or below 0.7 mega Pascals (MPa).
2. The system of claim 1 , wherein the at least one first solution comprises an acid, a base, a salt solution, or a combination thereof.
3. The system of claim 1 further comprising a control system configured to control operation of the at least one low pressure pump for supplying the at least one first solution.
4. The system of claim 3 , wherein the control system is configured to control a flow rate of the at least one first solution supplied by the at least one low pressure pump, or a flow rate of the high pressure pump, which determines concentration of the buffer solution.
5. The system of claim 1 , further comprising at least one sensor configured to measure characteristics of the buffer solution received at the high pressure pump.
6. The system of claim 5 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.
7. The system of claim 6 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.
8. The system of claim 1 , wherein the at least one valve includes a check valve.
9. The system of claim 1 , wherein the high pressure pump operates at a pressure from 0 to 20 mega Pascals (MPa).
10. The system of claim 1 , wherein the system does not comprise a pump for supplying the second solution to the T-joint.
11. A chromatography system comprising:
at least one chromatography column;
a T-joint for preparing a buffer solution by mixing at least one first solution and a second solution, wherein the T-joint receives the second solution from a solution supply unit connected to the T-joint, wherein the T-joint is connected to the solution supply unit using at least one direct connection tube, and wherein the T-joint is connected through at least one valve to a further solution supply unit for supplying the at least one first solution;
at least one low pressure pump for supplying the at least one first solution into the T-joint; and
a high pressure pump for collecting and delivering the buffer solution to the at least one chromatography column,
the chromatography system being characterized in that said T-joint includes a configuration for mixing the at least one first solution and the second solution, wherein the configuration for mixing the at least one first solution and the second solution is a magnetic stirrer,
wherein the system is configured such that the low-pressure pump, the T-joint, and the magnetic stirrer operate at a pressure of less than 0.7 mega Pascals (MPa).
12. The chromatography system of claim 11 , wherein the at least one first solution comprises an acid, a base, a salt solution, or a combination thereof.
13. The chromatography system of claim 11 , further comprising a control system configured to control operation of the at least one low pressure pump for supplying the at least one first solution and control a functioning of the T-joint to prepare the buffer solution.
14. The chromatography system of claim 13 , wherein the control system is configured to control a flow rate of the at least one first solution supplied by the at least one low pressure pump, or a flow rate of the high pressure pump, which determines concentration of the buffer solution.
15. The chromatography system of claim 11 , further comprising at least one sensor configured to measure characteristics of the buffer solution received at the high pressure pump.
16. The chromatography system of claim 15 , wherein a sensor of the at least one sensor is configured to measure a conductivity associated with the buffer solution.
17. The chromatography system of claim 15 , wherein a sensor of the at least one sensor is configured to measure a pH level associated with the buffer solution.
18. The chromatography system of claim 11 , wherein the at least one valve includes a check valve.
19. The chromatography system of claim 11 , wherein the high pressure pump operates at a pressure from 0 to 20 mega Pascals (MPa).
20. The chromatography system of claim 11 , wherein the system does not comprise a pump for supplying the second solution to the T-joint.
21. A system for performing inline blending or mixing of liquids in chromatography apparatus, the system comprising:
a T-joint for preparing a buffer solution by blending or mixing at least two initial solutions;
a liquid supply for each of the initial solutions, for supplying the respective initial solutions to the T-joint, one or more of the liquid supplies having a pump and one or more of the remaining liquid supplies having no pump, wherein the T-joint is connected to the one or more of the remaining liquid supplies by at least one direct connection tube, and wherein the T-joint is connected through at least one valve to the one or more of the liquid supplies having a pump; and
a liquid egress for removing the buffer solution from the T-joint, the liquid egress including a further pump operable at least to draw in solution from each of the liquid supplies which has no pump or the liquid supply which has a pump, and operable to deliver the buffer solution to a chromatography apparatus,
the system being characterized in that said T-joint includes a configuration for mixing the initial solutions, wherein the configuration for mixing the at least two initial solutions is a magnetic stirrer,
and wherein the system is configured such that the T-joint and the magnetic stirrer operate at a pressure of less than 0.7 mega Pascals (MPa).
22. The system of claim 21 , wherein the at least one valve includes a check valve.
23. The system of claim 21 , wherein the high pressure pump operates at a pressure from 0 to 20 mega Pascals (MPa).
24. The system of claim 21 , wherein the system does not comprise a pump for supplying a second of the initial solutions to the T-joint.