HIGH PURITY COPPER RADIOPHARMACEUTICAL COMPOSITIONS AND DIAGNOSTIC AND THERAPEUTIC USES THEREOF
The present disclosure relates to the field of nuclear imaging and therapy, and more specifically to high purity copper radiotracer compositions useful in imaging, such as positron emission tomography (PET) and single-photon emission computerized tomography (SPECT), and therapy. More specifically, the present disclosure relates to novel compositions useful in imaging and treatment of conditions such as prostate cancer, somatostatin receptor-expressing tumors, like neuroendocrine tumor, epithelial tumors, as well as to methods wherein such compositions are prepared.
1 . A composition comprising a radiotracer, the radiotracer comprising:
a chelating moiety;
a copper radionuclide (*Cu) chelated by the chelating moiety; and
a targeting moiety covalently linked to the chelating moiety;
wherein the composition is characterized by one or more of:
molar activity of ≥3 MBq/nmol;
radiochemical purity of ≥91%;
activity concentration of ≥8 MBq/mL; and
radionuclidic purity of the radiotracer of ≥95% at the end of synthesis.
2 . The composition according to claim 1 , wherein the radiotracer is of Formula X*:
or is a pharmaceutically acceptable salt thereof,
wherein:
is the chelating moiety;
*Cu is 61 Cu, 62 Cu, 64 Cu, or 67 Cu;
L is a bond or a linker moiety;
V is the targeting moiety;
n is an integer from 1 to 10;
m is an integer from 1 to 10; and
p is an integer from 1 to 10.
3 - 12 . (canceled)
13 . The composition according to claim 2 , wherein
is according to Formula II or II′:
wherein:
X 1 , X 2 , and X 3 are individually selected from —OH, —NH 2 , and —SH, including deprotonated variants thereof depending on chelation with *Cu;
any methylene is optionally substituted with oxo, thiol, or hydroxyl; and
denotes the point of attachment to the linker moiety (when L is a linker moiety) or to the targeting moiety (when L is a bond).
14 . (canceled)
15 . The composition according to claim 13 , wherein
is selected from Formula III, II′i, IIii, and IIiii:
16 - 17 . (canceled)
18 . The composition according to claim 15 , wherein the chelating moiety comprises: 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA); 2,2′-(7-(I-carboxy-4-oxopentyl)-1,4,7-triazonane-1,4-diyl)diacetic acid (NODAGA); 2-(4,7-bis(carboxymethyl)-1,4,7-triazonan-1-yl)succinic acid (NODASA); (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) (DOTA); (1,4,7,10-tetraazacyclododecane, 1-(glutaric acid)-4,7,10-triacetic acid) (DOTAGA); or (1,4,7,10-tetraazacyclododecane, 1-(succinic acid)-4,7,10-triacetic acid) (DOTASA).
19 . The composition according to claim 18 , wherein the chelating moiety comprises NOTA, NODASA, or NODAGA.
20 . The composition according to claim 19 , wherein the chelating moiety comprises NODAGA.
21 . The composition according to claim 18 , wherein *Cu is 61 Cu or 67 Cu.
22 - 23 . (canceled)
24 . The composition according to claim 21 , wherein the targeting moiety is recognized by a molecular target expressed by malignant or premalignant cells, cells in a tumor microenvironment, inflammatory tissues, or sites of tissue remodeling at sites of a myocardial infarct or fibrosis in interstitial lung disease.
25 . The composition according to claim 24 , wherein the molecular target is a tumor-specific antigen (TSA) or tumor-associated antigen (TAA).
26 . (canceled)
27 . The composition according to claim 18 , wherein the targeting moiety binds to prostate-specific membrane antigen (PSMA).
28 - 29 . (canceled)
30 . The composition according to claim 18 , wherein the targeting moiety binds to a somatostatin receptor (SSTR).
31 . (canceled)
32 . The composition according to claim 18 , wherein the targeting moiety binds to a fibroblast activation protein (FAP).
33 - 43 . (canceled)
44 . A method of generating an image of a subject, comprising:
administering to the subject a composition according to claim 1 , wherein *Cu is 61 Cu; and
generating an image of at least a part of the subject's body.
45 - 48 . (canceled)
49 . A method of detecting a disease in a subject, comprising:
administering to the subject the composition according to claim 1 , wherein *Cu is [ 61 Cu]Cu; and
detecting the localization of the radiotracer; and
determining the presence or absence of the disease based on the presence or absence of localization of the radiotracer.
50 - 57 . (canceled)
58 . A method of monitoring or determining the effect of cancer treatment on a subject afflicted with cancer, comprising:
administering to the subject the composition according to claim 1 , at an earlier time point, wherein *Cu is 61 Cu; and at a later time point;
detecting the localization of the radiotracer at both the earlier time point and at the later time point; and
monitoring or determining the effect of the cancer treatment by comparing the amount of localization at the later time point to the amount of localization at the earlier time point.
59 - 71 . (canceled)
72 . A method of making a composition according to claim 2 , the method comprising:
combining a copper radionuclide and a compound of Formula X:
or is a pharmaceutically acceptable salt thereof,
wherein:
is a chelating moiety:
L is a bond or a linker moiety;
V is the targeting moiety;
n is an integer selected from 1 to 10;
m is an integer selected from 1 to 10; and
p is an integer selected from 1 to 10.
73 - 74 . (canceled)
75 . The composition according to claim 2 , wherein the radiotracer is of Formula A*:
or is a pharmaceutically acceptable salt thereof,
wherein
n is 1; and
the chelating moiety comprises NODAGA.
76 . The composition according to claim 75 , wherein the targeting moiety binds to PSMA, SSTR, or FAP.
77 . The composition according to claim 2 , wherein *Cu is 61 Cu.
78 . The composition according to claim 77 , wherein the composition has a molar activity ≥8 MBq/nmol.
79 . The composition according to claim 78 , wherein the composition has a molar activity ≥20 MBq/nmol.
80 . The composition according to claim 77 , wherein the composition has a radiochemical purity of ≥95%.
81 . The composition according to claim 80 , wherein the composition has a radiochemical purity of ≥97%.
82 . The composition according to claim 77 , wherein the composition has an activity concentration of ≥8 MBq/mL.
83 . The composition according to claim 82 , wherein the composition has an activity concentration of ≥15 MBq/mL.
84 . The composition according to claim 77 , wherein the radiotracer has a radionuclidic purity characterized by one or more of the following:
110m Ag≤0.1 Bq/g;
108m Ag≤0.1 Bq/g; and
109 Cd≤0.1 Bq/g.
85 . The composition according to claim 77 , wherein the radiotracer has a chemical purity characterized by one or more of the following:
Fe≤2 μg/L;
69 Cu and 65 Cu together are ≤1 μg/L;
Zn≤2 μg/L;
Sn≤0.01 μg/L;
Ti≤0.01 μg/L;
Al≤2 μg/L;
As≤1 μg/L;
Ni≤1 μg/L; and
wherein any one of Cr, Cd, Co, and Y is ≤0.1 μg/mL.