IP Library › Granted Patent US 12,344,849
Granted Patent B2
US 12,344,849 · App. 18/481,023 · Granted Jul 1, 2025

INIR20 transgenic soybean with junction polynucleotide deletions

Inventors: Michael Lee Nuccio (Salem, NH); Michael Andreas Kock (Rheinfelden, DE); Joshua L. Price (Cambridge, MA)
Assignee: INARI AGRICULTURE TECHNOLOGY, INC.
C12N15/8201A01H1/02A01H5/10A01H6/4684A01H6/542C07K14/415C12N9/22C12N15/11C12N15/8213C12N15/8286C12Q1/6834C12Q1/6895C12N2310/20C12N2800/80C12Q2600/13C12Q2600/156C12Q2600/158
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,344,849
App. No.
18/481,023
Granted
Jul 1, 2025
Kind
B2
Abstract

Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Claims (1)

1. A biological sample from a transgenic soybean plant comprising a modified MON87701 transgenic locus, wherein the biological sample comprises the DNA molecule of SEQ ID NO: 2.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 6, 2023
From: NUCCIO, MICHAEL LEE; KOCK, MICHAEL ANDREAS; PRICE, JOSHUA L.
To: INARI AGRICULTURE TECHNOLOGY, INC.
Reel/Frame 065143/0750 →
Continuity (14)
Continuation 18058156 · Nov 22, 2022
Continuation PCTUS2021043933 · Jul 30, 2021
Provisional Application 63203137 · Jul 9, 2021
Provisional Application 63202569 · Jun 16, 2021
Provisional Application 63201030 · Apr 9, 2021
Provisional Application 63201029 · Apr 9, 2021
Provisional Application 63199949 · Feb 4, 2021
Provisional Application 63199951 · Feb 4, 2021
Provisional Application 63199930 · Feb 3, 2021
Provisional Application 63059860 · Jul 31, 2020
Provisional Application 63059813 · Jul 31, 2020
Provisional Application 63059916 · Jul 31, 2020
Provisional Application 63059963 · Jul 31, 2020
Related Publication 20240076683A1 · Mar 7, 2024
References Cited (129)
US 7632985B2 · Malven et al. · 2009 [cited by applicant]
US 7956246B2 · Bing et al. · 2011 [cited by applicant]
US 8049071B2 · Gao · 2011 [cited by examiner]
US 8232456B2 · Long et al. · 2012 [cited by applicant]
US 8450561B2 · Beazley et al. · 2013 [cited by applicant]
US 8455198B2 · Gao et al. · 2013 [cited by applicant]
US 8455720B2 · Long et al. · 2013 [cited by applicant]
US 8466346B2 · DeFramond et al. · 2013 [cited by applicant]
US 8575434B2 · Diehn et al. · 2013 [cited by applicant]
US 8680363B2 · Bard et al. · 2014 [cited by applicant]
US 9540655B2 · Cui et al. · 2017 [cited by applicant]
US 10093940B2 · Sastry-Dent et al. · 2018 [cited by applicant]
US 10260062B2 · Ainley et al. · 2019 [cited by applicant]
US 10538774B2 · D'Halluin · 2020 [cited by applicant]
US 11041172B2 · Cermak et al. · 2021 [cited by applicant]
US 11214811B1 · Nuccio et al. · 2022 [cited by applicant]
US 11242534B1 · Nuccio et al. · 2022 [cited by applicant]
US 11359210B2 · Price et al. · 2022 [cited by applicant]
US 11814630B2 · Price et al. · 2023 [cited by applicant]
US 11814631B2 · Nuccio et al. · 2023 [cited by applicant]
US 20100162428A1 · Brown et al. · 2010 [cited by applicant]
US 20110191899A1 · Ainley et al. · 2011 [cited by applicant]
US 20140196169A1 · D'Halluin et al. · 2014 [cited by applicant]
US 20150059010A1 · Cigan et al. · 2015 [cited by applicant]
US 20150082478A1 · Cigan et al. · 2015 [cited by applicant]
US 20160333363A1 · Srivastava · 2016 [cited by applicant]
US 20170166912A1 · Brower-Toland et al. · 2017 [cited by applicant]
US 20180057878A1 · Bing et al. · 2018 [cited by applicant]
US 20180163218A1 · Corbin et al. · 2018 [cited by applicant]
US 20190112614A1 · Russell et al. · 2019 [cited by applicant]
US 20190136249A1 · Sakai et al. · 2019 [cited by applicant]
US 20190345512A1 · Moser et al. · 2019 [cited by applicant]
US 20190352655A1 · Niu et al. · 2019 [cited by applicant]
US 20200157554A1 · Cigan et al. · 2020 [cited by applicant]
US 20200405649A1 · Wang et al. · 2020 [cited by applicant]
US 20220030806A1 · Price et al. · 2022 [cited by applicant]
US 20220030822A1 · Nuccio et al. · 2022 [cited by applicant]
US 20220033833A1 · Gilbertson et al. · 2022 [cited by applicant]
US 20220033836A1 · Price et al. · 2022 [cited by applicant]
US 20220098602A1 · Nuccio et al. · 2022 [cited by applicant]
US 20220154194A1 · Nuccio et al. · 2022 [cited by applicant]
US 20220251584A1 · Nuccio et al. · 2022 [cited by applicant]
US 20220364105A1 · Price et al. · 2022 [cited by applicant]
US 20230077473A1 · Price et al. · 2023 [cited by applicant]
US 20230078387A1 · Kock et al. · 2023 [cited by applicant]
US 20230083144A1 · Nuccio et al. · 2023 [cited by applicant]
US 20230087222A1 · Kock et al. · 2023 [cited by applicant]
US 20230147013A1 · Nuccio et al. · 2023 [cited by applicant]
US 20230313221A1 · Kock et al. · 2023 [cited by applicant]
US 20240011043A1 · Kock et al. · 2024 [cited by applicant]
WO 2010077816A1 · 2010 [cited by applicant]
WO 2022026375A1 · 2022 [cited by applicant]
WO 2022026379A1 · 2022 [cited by applicant]
WO 2022026390A1 · 2022 [cited by applicant]
WO 2022026395A2 · 2022 [cited by applicant]
WO 2022026403A2 · 2022 [cited by applicant]
WO 2022026801A1 · 2022 [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/302,739, 24 pages, mailed Aug. 3, 2021. [cited by applicant]
Notice of Allowance in U.S. Appl. No. 17/249,640, 7 pages, mailed Sep. 22, 2021. [cited by applicant]
Que et al., “Maize transformation technology development for commercial event generation”, Frontiers in Plant Science, vol. 5, Article 379, pp. 1-19, 2014. [cited by applicant]
Srivastava et al., “Gene Stacking by recombinases”, Plant Biotechnology Journal, vol. 14, pp. 471-482, 2016. [cited by applicant]
Srivastava, et al., “Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome”, Plant Cell Tissue and Organ Culture, vol. 129, pp. 153-160, 2017. [cited by applicant]
Ward et al., “Petition for Determination of Nonregulated Status for Insect-Resistant MIR162 Maize”, Syngenta, pp. 1-271, 2007. [cited by applicant]
Xing et al., “Revealing frequent alternative polyadenylation and widespread low-level transcription read-through of novel plant transcription terminators”, Plant Biotechnology Journal, vol. 8, pp. 772-782, 2010. [cited by applicant]
Young et al., “CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement”, Scientific Reports, vol. 9, 11 pages, Apr. 15, 2019. [cited by applicant]
“What is a CRISPR-Cas System?”, CRISPR-Cas++, https://crisprcas.i2bc. paris-saclay.fr/Home/About, 2 pages, accessed Nov. 2, 2021. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/162,134, 28 pages, mailed Jun. 21, 2023. [cited by applicant]
Danilo et al., “The DFR locus: A smart landing pad for targeted transgene insertion in tomato,” PLoS One, vol. 13, No. 12, 14 pages, Dec. 6, 2018. [cited by applicant]
Eaglesham et al., “New DNA-Editing Approaches: Methods, Applications & Policy for Agriculture,” North American Agricultural Biotechnology Council Report, NABC Report 26, 255 pages, 2014. [cited by applicant]
Gleditzsch et al., “PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures,” RNA Biology, vol. 16, No. 4, pp. 504-517, 2019. [cited by applicant]
Rudgers et al., “EXZACTTM Precision Technology: Scientific and Regulatory Advancements in Plant-Genome Editing with ZFNs,” North American Biotechnology Council, pp. 113-124, 2014. [cited by applicant]
Shi et al., “ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions,” Plant Biotechnology Journal, vol. 15, pp. 207-216, 2017. [cited by applicant]
Yau et al., “Less is more: strategies to remove marker genes from transgenic plants,” BMC Biotechnology, vol. 13, No. 36, 23 pages, 2013. [cited by applicant]
Office Action in U.S. Appl. No. 18/058,081, 27 pages, mailed Apr. 11, 2023. [cited by applicant]
Office Action in U.S. Appl. No. 18/058,161, 23 pages, mailed Apr. 11, 2023. [cited by applicant]
Begemann et al., “Precise insertion and guided editing of higher plant genomes using Cpf1 CRISP nucleases,” Scientific Reports, vol. 7, No. 11606, pp. 1-6, Sep. 14, 2017. [cited by applicant]
Begemann et al., Supplementary Data—Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases, Scientific Reports, vol. 7, No. 11606, 18 pages, Sep. 14, 2017. [cited by applicant]
International Search Report in PCT/US2021/043161, 6 pages, mailed Jan. 5, 2022. [cited by applicant]
International Search Report in PCT/US2021/043170, 6 pages, mailed Jan. 5, 2022. [cited by applicant]
International Search Report in PCT/US2021/043187, 6 pages, mailed Jan. 6, 2022. [cited by applicant]
International Search Report in PCT/US2021/043192, 7 pages, mailed Jan. 27, 2022. [cited by applicant]
International Search Report in PCT/US2021/043207, 6 pages, mailed Jan. 27, 2022. [cited by applicant]
International Search Report in PCT/US2021043851, 6 pages, Dec. 30, 2021. [cited by applicant]
International Search Report in PCT/US2021/043919, 8 pages, mailed Jan. 20, 2022. [cited by applicant]
International Search Report in PCT/US2021/043933, 6 pages, mailed Dec. 30, 2021. [cited by applicant]
International Search Report in PCT/US2021/044198, 6 pages, mailed. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/302,110, 27 pages, mailed May 24, 2023. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/650,031, 11 pages, mailed May 26, 2023. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/680,647, 11 pages, mailed Jun. 23, 2022. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/057,860, 49 pages, mailed Jun. 1, 2023. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/057,867, 17 pages, mailed Jun. 7, 2023. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/058,144, 49 pages, mailed Jun. 7, 2023. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/058,156, 24 pages, mailed May 19, 2023. [cited by applicant]
Zhang et al., “Off-target Effects in CRISPR/Cas9-mediated Genome Engineering,” Molecular Therapy—Nucleic Acids, vol. 4, pp. 1-8, Nov. 17, 2015. [cited by applicant]
Zhong et al., “Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites,” Molecular Plant, vol. 11, No. 7, pp. 999-1002, Jul. 2018. [cited by applicant]
Zhong et al., “Supplementary Data—Plant Genome Editing Using FnCpf1 and LbCpf1 Nucleases at Redefined and Altered PAM Sites,” Molecular Plant, vol. 11, No. 7, 36 pages, Jul. 2018. [cited by applicant]
Baliga et al., “Investigation of direct repeats, spacers and proteins associated with clustered regularly interspaced short palindromic repeat (CRISPR) system of Vibrio parahaemolyticus”, Molecular Genetics and Genomics… [cited by applicant]
Biopesticides Registration Action Document, “Bacillus thuringiensis Vip3Aa20 Insecticidal Protein and the Genetic Material Necessary for Its Production (via Elements of Vector pNOV1300) in Event MIR162 Maize (OECD Uniqu… [cited by applicant]
Bissler, John J., “Triplex DNA and Human Disease”, Frontiers in Bioscience, vol. 12, pp. 4536-4546, May 1, 2007. [cited by applicant]
Bortesi et al., “The CRISPR/Cas9 system for plant genome editing and beyond”, Biotechnology Advances, vol. 33, pp. 41-52, available online Dec. 20, 2014. [cited by applicant]
Charpentier et al., “Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity”, FEMS Microbiology Reviews, fuv023, vol. 39, pp. 428-441, 2015. [cited by applicant]
Cho et al., “Nonallelic homologous recombination events responsible for copy number variation within an RNA silencing locus”, Plant Direct, vol. 3, 16 pages, Aug. 5, 2019. [cited by applicant]
Du et al., “Construction of Marker-Free Genetically Modified Maize Using a Heat-Inducible Auto-Excision Vector”, Genes, vol. 10, No. 374, pp. 1-17, 2019. [cited by applicant]
Du et al., “Infection of Embryonic Callus with Agrobacterium Enables High-Speed Transformation of Maize”, International Journal of Molecular Sciences, vol. 20,. No. 279, pp. 1-15, doi:10.3390/ijms20020279, 2019. [cited by applicant]
Finnigan et al., “mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence”, G3, vol. 6, pp. 2147-2156, Jul. 2016. [cited by applicant]
Gurusaran et al., “RepEx: Repeat extractor for biological sequences”, Genomics, vol. 102, pp. 403-408, 2013. [cited by applicant]
International Searching Authority in connection with PCT/US21/43897 filed Jul. 30, 2021, “Invitation to Pay Additional Fees And, Where Applicable, Protest Fee”, 3 pages, mailed Oct. 27, 2021. [cited by applicant]
International Searching Authority in connection with PCT/US21/43935 filed Jul. 30, 2021, “Invitation to Pay Additional Fees And, Where Applicable, Protest Fee”, 3 pages, mailed Oct. 26, 2021. [cited by applicant]
International Searching Authority in connection with PCT/US21/43945 filed Jul. 30, 2021, “Invitation to Pay Additional Fees And, Where Applicable, Protest Fee”, 4 pages, mailed Oct. 27, 2021. [cited by applicant]
Kim et al., “CRISPR/Cpf1-mediated DNA-free plant genome editing”, Nature Communications, vol. 8, Article No. 14406, 7 pages, Feb. 16, 2017. [cited by applicant]
Li et al., “Expanding the Scope of CRISPR/Cpf1-Mediated Genome Editing in Rice”, Molecular Plant, vol. 11, No. 7, pp. 1-14, 2018. [cited by applicant]
Luo et al., “Improperly Terminated, Unpolyadenylated mRNA of Sense Transgenes is Targeted by RDR6-Mediated RNA Silencing in [cited by applicant]
Malzahn et al., “Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/248,936, 25 pages, mailed Mar. 25, 2021. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/249,640, 10 pages, mailed Jun. 29, 2021. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/302,110, 22 pages, mailed Jun. 29, 2021. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 17/302,121, 10 pages, mailed Jul. 8, 2021. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/452,225, mailed Jun. 20, 2024, 42 pages. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/452,330, mailed Jun. 20, 2024, 44 pages. [cited by applicant]
Non-Final Office Action in U.S. Appl. No. 18/481,069, mailed Jun. 20, 2024, 21 pages. [cited by applicant]
Syngenta, “5307 Maize Syngenta Agrisure Duracade,” Information for Operators, Updated Dec. 2020, 5 pages. [cited by applicant]
United States Environmental Protection Agency, “Non-PRIA Labeling and Formulation Amendment,” Plant-Incorporated Protectant Label for Herculex RW Insect Protection, Sep. 25, 2015, 5 pages. [cited by applicant]
Office Action in U.S. Appl. No. 18/452,225, mailed Nov. 19, 2024, 20 pages. [cited by applicant]
Office Action in U.S. Appl. No. 17/806,435, mailed Nov. 18, 2024, 33 pages. [cited by applicant]
Office Action in U.S. Appl. No. 18/481,069, mailed Nov. 19, 2024, 21 pages. [cited by applicant]
Office Action in U.S. Appl. No. 18/481,358, mailed Nov. 19, 2024, 20 pages. [cited by applicant]
Marques et al. “Field evaluation of soybean transgenic event DAS-81419-2 expressing Cry1F and Cry1Ac proteins for the control of secondary lepidopteran pests in Brazil.” Crop Protection 96 (2017): 109-115. [cited by applicant]
Zhao et al., “An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design,” Scientific Reports, 2016, vol. 6, No. 23890, 1-11. [cited by applicant]
Zhao et al., “An alternative strategy for targeted gene replacement in plants using a dual-sgRNA/Cas9 design,” Supplementary Materials Information, Scientific Reports, 2016, vol. 6, No. 23890, 32 pages. [cited by applicant]