Flow cell receiver and methods of use
The present disclosure relates to a flow cell receiver. The flow cell receiver can include at least one platen, having a plurality of ports. The flow cell receiver can include magnets. The flow cell receiver can be configured to automatically align, secure, and retain a flow cell carrier containing a flow cell.
1. A method of detecting a fluorescently labeled nucleotide in a sample using a receiving device comprising a platform, a vacuum port and a magnet, wherein the vacuum port and the magnet are configured to align, secure, and retain a solid support on the platform, said method comprising:
placing a solid support comprising a sample on the platform, wherein the sample comprises the fluorescently labeled nucleotide;
directing an excitation beam onto the sample; and
detecting the fluorescently labeled nucleotide in the sample by detecting a light emission from the sample.
2. The method of claim 1 , wherein the receiving device further comprises a plurality of vacuum ports.
3. The method of claim 1 , wherein the vacuum port is configured to provide sufficient vacuum pressure to ensure a maximum physical contact between the solid support and the platform.
4. The method of claim 1 , wherein the receiving device further comprises a fluidic system configured to provide one or more fluids to the solid support.
5. The method of claim 1 , further comprising providing a constructive interference with the magnet by orienting the magnet.
6. The method of claim 1 , wherein the vacuum port and the magnet prevents a significant movement of the solid support.
7. The method of claim 1 , wherein the platform further comprises a visible light absorbing coating, or an ultraviolet light absorbing coating, or an infrared light absorbing coating, or a combination thereof.
8. The method of claim 1 , wherein the receiving device further comprises a thermoelectric heating element configured to modulate temperature of the platform.
9. The method of claim 8 , wherein the thermoelectric heating element is a Peltier device.
10. The method of claim 3 , wherein the sufficient vacuum pressure is less than about 760 torr.
11. The method of claim 1 , wherein said receiving device further comprises a circuit board.
12. The method of claim 11 , wherein the solid support further comprises an electronically erasable programmable read only memory chip, and the circuit board is configured to contact to the electronically erasable programmable read only memory chip.
13. The method of claim 11 , wherein said circuit board is configured to store information and to process the information, and/or to modulate a radio-frequency signal and demodulate the radio-frequency signal.
14. The method of claim 1 , wherein the fluorescently labeled nucleotide comprises a 3′ reversible terminator.
15. The method of claim 1 , wherein the fluorescently labeled nucleotide forms part of a nucleic acid molecule.
16. The method of claim 15 , wherein the nucleic acid molecule is hybridized to a target polynucleotide.
17. The method of claim 1 , wherein the sample further comprises a plurality of fluorescently labeled nucleotides.
18. The method of claim 17 , further comprising adjusting a position of the solid support using the platform, and detecting a second fluorescently labeled nucleotide in the sample.
19. The method of claim 1 , wherein the sample comprises four fluorescently labeled nucleotides, wherein each of the four fluorescently labeled nucleotides comprises a different fluorescent label.
20. The method of claim 1 , wherein the receiving device further comprises an input port and an output port.