Granted Patent B2

US 12,644,886 · App. 18/489,398 · Granted Jun 2, 2026

Inventors: Joshua LaBaer (Chandler, AZ); Bharath Takulapalli (Chandler, AZ)

Assignee: Arizona Board of Regents on behalf of Arizona State University

G01N33/54373G01N2440/00

Abstract

Claims (14)

1 . A method for generating a biosensor microarray, comprising:

(i) providing a plurality of capture moieties linked to a first solid support substrate surface;

(ii) providing an array of separate nanowells or microwells, in which in vitro translation reactions are performed, on a second solid support substrate surface, each nanowell or microwell comprising RNAs encoding detector polypeptides, ribosomes, and detector polypeptides translated from the RNAs;

(iii) providing a plurality of sensors in direct contact with or at a distance within about 1 millimeter (mm) of the detector polypeptides;

(iv) contacting the array with the plurality of capture moieties, whereby the detector polypeptides bind specifically to the capture moieties; and

(v) washing the contacted capture moieties to remove in vitro translation contaminants that are non-specifically bound to the capture moieties, whereby a biosensor microarray substantially free of in vitro translation contaminants is obtained.

2 . The method of claim 1 , wherein the array comprises at least 100 nanowells or microwells in which in vitro translation reactions are performed, wherein each nanowell or microwell comprises a translated detector polypeptide with a different amino acid sequence from the amino acid sequences of the other translated detector polypeptides.

3 . The method of claim 1 , wherein the capture moieties are provided as a monolayer.

4 . The method of claim 1 , wherein the array comprises sealable nanowells.

5 . The method of claim 4 , wherein each nanowell is about 250 microns in diameter and about 75 microns deep.

6 . The method of claim 5 , wherein each nanowell comprises a center to center distance between the nanowells of at least 400 nm to about 1,000 nm.

7 . The method of claim 1 , wherein the array comprises microwells.

8 . The method of claim 7 , wherein each microwell is between about 300 nm to about 1,000 nm in diameter.

9 . The method of claim 1 , wherein the array is on silicon wafers.

Assignments (1)

Continuity (4)

References Cited (70)