Vaccines For Human Papilloma Virus And Methods For Using The Same
Improved anti-HPV immunogens and nucleic acid molecules that encode them are disclosed. Immunogens disclosed include those having consensus HPV39 E6E7 and HPV45 E6E7. Pharmaceutical composition, recombinant vaccines comprising DNA plasmid and live attenuated vaccines are disclosed as well methods of inducing an immune response in an individual against HPV are disclosed.
1 .- 9 . (canceled)
10 . A composition comprising at least one HPV52 E6-E7 fusion antigen; wherein the HPV52 E6-E7 fusion antigen is selected from the group consisting of SEQ ID NO:20, a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:20, a peptide comprising a fragment of SEQ ID NO:20, a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:20.
11 . The composition of claim 10 , further comprising at least one additional HPV E6-E7 fusion antigen selected from the group consisting of:
SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO: 8, SEQ ID NO:18, SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26;
a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:18; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:22; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:24; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:25; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:26;
a peptide comprising a fragment of SEQ ID NO:2; a peptide comprising a fragment of SEQ ID NO:4; a peptide comprising a fragment of SEQ ID NO:6; a peptide comprising a fragment of SEQ ID NO:8; a peptide comprising a fragment of SEQ ID NO:18; a peptide comprising a fragment of SEQ ID NO:22; a peptide comprising a fragment of SEQ ID NO:24; a peptide comprising a fragment of SEQ ID NO:25; a peptide comprising a fragment of SEQ ID NO:26;
a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO: 18; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:22; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:24; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:25; and a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:26.
12 . The composition of claim 11 , wherein at least one additional HPV E6-E7 fusion antigen is selected from the group consisting of:
SEQ ID NO:2; SEQ ID NO:4; SEQ ID NO:6; SEQ ID NO:8; SEQ ID NO:18; SEQ ID NO:22; SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:18; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:22; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:24;
a peptide comprising a fragment of SEQ ID NO:2; a peptide comprising a fragment of SEQ ID NO:4; a peptide comprising a fragment of SEQ ID NO:6; a peptide comprising a fragment of SEQ ID NO:8; a peptide comprising a fragment of SEQ ID NO:18; a peptide comprising a fragment of SEQ ID NO:22; a peptide comprising a fragment of SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO: 18; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:22; and a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:24.
13 . The composition of claim 11 , wherein at least one additional HPV E6-E7 fusion antigen is selected from the group consisting of:
SEQ ID NO:6; SEQ ID NO:8; SEQ ID NO: 18; SEQ ID NO:22; SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:18; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:22; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:24;
a peptide comprising a fragment of SEQ ID NO:6; a peptide comprising a fragment of SEQ ID NO:8; a peptide comprising a fragment of SEQ ID NO:18; a peptide comprising a fragment of SEQ ID NO:22; a peptide comprising a fragment of SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:6; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:8; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO: 18; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:22; and a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:24.
14 . The composition of claim 11 , wherein one or more additional HPV E6-E7 fusion antigen is selected from the group consisting of:
SEQ ID NO:2; SEQ ID NO:4; SEQ ID NO:22; SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:22; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:24;
a peptide comprising a fragment of SEQ ID NO:2; a peptide comprising a fragment of SEQ ID NO:4; a peptide comprising a fragment of SEQ ID NO:22; a peptide comprising a fragment of SEQ ID NO:24;
a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:2; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:4; a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:22; and a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:24.
15 . The composition of claim 11 , wherein at least one additional HPV E6-E7 fusion antigen is selected from the group consisting of:
SEQ ID NO:18; SEQ ID NO:22;
a peptide comprising a sequence that is at least 95% identical to SEQ ID NO: 18; a peptide comprising a sequence that is at least 95% identical to SEQ ID NO:22;
a fragment of a nucleotide sequence that encodes SEQ ID NO:18; a fragment of a nucleotide sequence that encodes SEQ ID NO:22;
a peptide comprising a fragment of SEQ ID NO: 18; a peptide comprising a fragment of SEQ ID NO:22;
a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:18; and a peptide comprising a sequence that is at least 95% identical to a fragment of SEQ ID NO:22.
16 . The composition of claim 10 , wherein the HPV52 E6-E7 fusion antigen is at least 98% identical to SEQ ID NO:20.
17 . The composition of claim 10 , wherein the HPV52 E6-E7 fusion antigen is at least 99% identical to SEQ ID NO:20.
18 . The composition of claim 10 , wherein the fragment of SEQ ID NO:20 comprises a the fragment of SEQ ID NO:20 without the leader sequence of SEQ ID NO:10.
19 . The composition of claim 10 , wherein the composition is a pharmaceutical composition.
20 . A method of inducing an effective immune response in an individual against more HPV52, comprising administering to said individual a composition of claim 10 .
21 . A method of inducing an effective immune response in an individual against more than one subtype of HPV, comprising administering to said individual a composition of claim 11 .