VECTORS

1 . A kit of vectors for transducing an immune cell with multiple transgenes, the kit comprising:

(i) a first vector which comprises a first transgene and a nucleotide sequence encoding a transcription factor; and

(ii) a second vector which comprises a second transgene

wherein expression of the second transgene within a host cell is dependent upon expression of the transcription factor.

2 . A kit of vectors according to claim 1 , comprising:

(i) a first vector which comprises a first transgene and a nucleotide sequence encoding a transcription factor;

(ii) a second vector which comprises a second transgene; and

(iii) a third vector which comprises a third transgene

wherein expression of the second and third transgenes within a host cell is dependent upon expression of the transcription factor.

3 . A kit of vectors according to claim 1 , comprising:

(i) a first vector which comprises a first transgene and a nucleotide sequence encoding a first transcription factor;

(ii) a second vector which comprises a second transgene and a nucleotide sequence encoding a second transcription factor; and

(iii) a third vector which comprises a third transgene

wherein expression of the second transgene within a host cell is dependent upon expression of the first transcription factor, and expression of the third transgene within a host cell is dependent upon expression of the second transcription factor.

4 . A kit of vectors according to claim 1 , comprising:

(i) a first vector which comprises a first transgene and a nucleotide sequence encoding a first transcription factor; and

(ii) a second vector which comprises a second transgene and a nucleotide sequence encoding a second transcription factor;

wherein expression of the second transgene within a host cell is dependent upon expression of the first transcription factor, and expression of the first transgene within a host cell is dependent upon expression of the second transcription factor.

5 . A kit according to claim 1 , wherein one or more of the vector(s) also comprises a marker gene.

6 . A kit according to claim 1 , wherein the vectors are plasmids, transposons, retroviral vectors or lentiviral vectors.

7 . A kit according to claim 1 wherein the or each transcription factor is from a non-immune cell or is an artificial transcription factor.

8 . A kit according to claim 1 , wherein the first vector comprises a constitutively active promoter.

9 . A kit according to claim 1 , wherein one or more of the transgene(s) encodes a chimeric antigen receptor (CAR) or a T-cell receptor (TCR).

10 . A cell which comprises a transgene and a nucleotide sequence encoding a non-endogenous transcription factor, wherein expression of the transgene is dependent upon expression of the non-endogenous transcription factor.

11 . A cell transfected or transduced with a kit of vectors according to claim 1 .

12 . A cell according to claim 11 , which is an immune cell.

13 . A cell according to claim 12 , which is a T-cell or natural killer (NK) cell.

14 . A method for making a cell which comprises the step of transducing or transfecting a cell with a kit of vectors according to claim 1 .

15 . A method for making a cell composition expressing a kit of vectors according to claim 1 , which comprises the following steps:

(i) transducing or transfecting a cell with a kit of vectors according to claim 1 wherein one of the vectors comprises a marker gene;

(ii) detecting expression of the marker gene; and

(iii) selecting cells which express the marker gene

wherein expression of the marker gene by a cell indicates that the comprises all of the vectors in the kit

16 . A method according to claim 14 , wherein the cell(s) is/are transduced or transfected ex vivo.

17 . A pharmaceutical composition comprising a plurality of cells according claim 11 .

18 . A pharmaceutical composition according to claim 17 for use in treating and/or preventing a disease.

19 . A method for treating a disease, which comprises the step of administering a pharmaceutical composition according to claim 17 to a subject.

20 . A method for treating a disease, which comprises the following steps:

(i) isolation of a cell containing sample from a subject with the disease;

(ii) transduction or transfection of the cells with a kit of vectors according to claim 1 ; and

(iii) administering the cells from step (ii) to the subject.

21 - 22 . (canceled)