Composition for regulating production of interfering ribonucleic acid
The embodiments of the present disclosure relate to decreasing the bioavailability of one or more target biomolecules by providing a composition that comprises a recombinant plasmid with one or more sequences of micro interfering ribonucleic acid (miRNA). When the recombinant plasmid interacts with a target cell, it causes the target cell to upregulate production of the miRNA, which then decreases the bioavailability of the target biomolecule. In some embodiments of the present disclosure, the target biomolecule is a protein that participates in one or more intracellular processes.
1. A composition that comprises a recombinant plasmid (RP) with a sequence of nucleotides according to SEQ ID NO: 1 that comprise a start region, an end region and an insert positioned between the start region and the end region, in which the insert encodes for an insert sequence of micro interfering ribonucleic acid (miRNA) that binds to and causes degradation of messenger ribonucleic acid (mRNA) that encodes for a target biomolecule, wherein the target biomolecule participated in one or more intra-cellular processes, wherein the insert sequence comprises 95-100% of a same nucleotide sequence as one of SEQ ID NO: 2, SEQ ID NO: 3; and SEQ ID NO: 4.
2. The composition of claim 1 , wherein the sequence of nucleotides is configured to be delivered to a target cell that has an over-expressed or mis-expressed biomolecule, wherein the sequence of nucleotides is encased in a protein coat, a lipid vesicle, or any combination thereof.
3. The composition of claim 1 wherein: when the insert sequence comprises 95-100% of the same nucleotide sequence as SEQ ID NO: 2 the target molecule is MRS2, when the insert sequence comprises 95-100% of the same nucleotide sequence as SEQ ID NO: 3 the target molecule is DUX4, and when the insert sequence comprises 95-100% of the same nucleotide sequence as SEQ ID NO: 4, the target molecule is tyrosine hydroxylase.
4. The composition of claim 1 , wherein the sequence of nucleotides is configured to be delivered to a target cell that has an over-expressed or mis-expressed biomolecule, wherein the sequence of nucleotides is encased in a virus vector.
5. The composition of claim 4 , wherein the virus vector is one of a double stranded DNA virus, a single stranded DNA virus, a single stranded RNA virus, or a double stranded RNA virus.
6. The compositions of claim 4 , wherein the virus vector is an adeno-associated virus.