IP Library Granted Patent US 12,344,828
Granted Patent B2
US 12,344,828 · App. 18/542,048 · Granted Jul 1, 2025

Cell-based meat products and cell biomasses with cells cultivated in a filter cake

Inventor: Konrad Mueller-Auffermann (Emeryville, CA)
Assignee: Upside Foods, Inc.
C12M25/02C12M29/10C12M45/02
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Quick Facts
Patent No.
US 12,344,828
App. No.
18/542,048
Granted
Jul 1, 2025
Kind
B2
Abstract

Provided herein are filter cake-based systems and methods for cultivating cells and cell biomass therefrom. Provided herein is a system for cultivating cells and cell biomass comprising a filter chamber comprising at least one inlet and at least one outlet, at least one filter support located within the filter chamber, and a filter cake located on the filter support, wherein the filter cake comprises at least one filter aid and a plurality of cells. Provided herein is a method for optimizing the cultivation of cells and cell biomass, comprising providing a filter support, adding at least one filter aid to the filter support, adding a plurality of cells to the filter aid, wherein the cells and the filter aid together comprise a filter cake, growing the cells into a cell biomass in the filter cake, wherein the filter cake is at least partially compressible.

Claims (27)

1. A cell-based meat product comprising:

an edible filter aid comprising a plurality of discrete and separate particles or fibers; and

a plurality of cells, the plurality of cells being derived from a non-human animal species intended for human or non-human dietary consumption;

wherein cells of the plurality of cells are embedded within the edible filter aid.

2. The cell-based meat product as in claim 1 , wherein the edible filter aid comprises one or more of fungal mycella, plant fibers, or cellulose.

3. The cell-based meat product as in claim 1 , wherein the edible filter aid comprise one or more of algae, plant fibers, starch particles, or plant-based proteins.

4. The cell-based meat product as in claim 1 , wherein the edible filter aid is hollow.

5. The cell-based meat product as in claim 1 , wherein the edible filter aid is degradable.

6. The cell-based meat product as in claim 5 , wherein the edible filter aid is configured to be at least partially degraded by cell secretions.

7. The cell-based meat product as in claim 1 , wherein the edible filter aid is compressible.

8. The cell-based meat product as in claim 1 , wherein cells of the plurality of cells adhere to the edible filter aid.

9. The cell-based meat product as in claim 1 , wherein the plurality of cells comprises:

cells of a first type comprising one of myoblasts, adipocytes, or fibroblasts; and

cells of a second type comprising another of myoblasts, adipocytes, or fibroblasts.

10. The cell-based meat product as in claim 9 , wherein the cells of the first type are arranged in a first layer and the cells of the second type are arranged in an adjacent layer.

11. A cell biomass comprising:

an edible filter aid comprising a plurality of discrete and separate particles or fibers; and

a plurality of cells grown about the edible filter aid, the plurality of cells being derived from a non-human animal species intended for human or non-human dietary consumption.

12. The cell biomass of claim 11 , wherein the edible filter aid comprises one or more of fungal mycella, plant fibers, or cellulose.

13. The cell biomass of claim 11 , wherein the cell biomass is a meat product for human dietary consumption.

14. The cell biomass of claim 11 , wherein the edible filter aid comprise one or more of algae, plant fibers, starch particles, or plant-based proteins.

15. The cell biomass of claim 11 , wherein the edible filter aid is degradable.

16. The cell biomass of claim 11 , wherein the plurality of cells comprise cells from a species of poultry, game, aquatic, or livestock.

17. The cell biomass of claim 11 , wherein the edible filter aid is hollow.

18. The cell biomass of claim 11 , wherein the edible filter aid comprises fibers with a fiber titer of between about 0.01 dtex-about 120 dtex, about 0.05 dtex-about 60 dtex, about 0.1 dtex-about 30 dtex, about 0.2 dtex-about 15 dtex, or about 0.5 dtex-about 5 dtex.

19. The cell biomass of claim 11 , wherein the edible filter aid comprises fibers of a length of between about 1 μm-about 20 μm, about 20 μm-about 50 μm, about 50 μm-about 80 μm, about 80 μm-about 110 μm, about 110 μm-about 140 μm, about 140 μm-about 170 μm, about 170 μm-about 200 μm, about 200 μm-about 230 μm, about 230 μm-about 260 μm, about 260 μm-about 290 μm, or about 290 μm-about 320 μm.

20. The cell biomass of claim 11 , wherein cells of the plurality of cells adhere to the edible filter aid.

Assignments (2)
CHANGE OF NAME Recorded May 27, 2026
From: UPSIDE FOODS, INC.
To: THE UPSIDE GROUP INC.
Reel/Frame 075648/0897 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 18, 2023
From: MUELLER-AUFFERMANN, KONRAD
To: UPSIDE FOODS, INC.
Reel/Frame 065895/0166 →
Continuity (3)
Continuation 17656986 · Mar 29, 2022
Continuation 17481176 · Sep 21, 2021
Related Publication 20240117287A1 · Apr 11, 2024
References Cited (39)
US 5501971A · Freedman et al. · 1996 [cited by applicant]
US 8703216B2 · Forgacs · 2014 [cited by examiner]
US 9101093B2 · Chance · 2015 [cited by examiner]
US 11319520B1 · Mueller-Auffermann · 2022 [cited by examiner]
US 11891596B2 · Mueller-Auffermann · 2024 [cited by examiner]
US 20050244943A1 · Ladisch et al. · 2005 [cited by applicant]
US 20090304677A1 · Ichim et al. · 2009 [cited by applicant]
US 20140248698A1 · Kotera et al. · 2014 [cited by applicant]
US 20200056144A1 · Castillo et al. · 2020 [cited by applicant]
US 20200080050A1 · Nahmias · 2020 [cited by examiner]
US 20200224144A1 · Love et al. · 2020 [cited by applicant]
US 20200248120A1 · Ferrie et al. · 2020 [cited by applicant]
US 20210145031A1 · Leung et al. · 2021 [cited by applicant]
US 20220195359A1 · Lavon · 2022 [cited by examiner]
JP 2019068760A · 2019 [cited by applicant]
KR 1020130110144A · 2013 [cited by applicant]
WO 2013058403A1 · 2013 [cited by applicant]
WO 2015130222A1 · 2015 [cited by applicant]
WO 2018170139A1 · 2018 [cited by applicant]
WO 2021108093A1 · 2021 [cited by applicant]
WO 2021111270A1 · 2021 [cited by applicant]
Extended European Search Report as received in EP application 22873757.3 dated Jun. 24, 2024. [cited by applicant]
Notice of Acceptance as received in AU 2024202654 dated Jun. 4, 2024. [cited by applicant]
Office Action as received in CN application 202280029807.0 dated Jul. 16, 2024. [cited by applicant]
Notice of Allowability as received in IL application 308441 dated May 9, 2024. [cited by applicant]
International Preliminary Report on Patentability as received in PCT/US2022/071418, dated Apr. 4, 2024. [cited by applicant]
Notice of Acceptance received in AU application 2022349541, dated Jan. 8, 2024. [cited by applicant]
Office Action as received in CN application 202280029807.0, dated Feb. 21, 2024. [cited by applicant]
International Search Report & Written Opinion as received in PCT/US2022/071418 dated Jul. 8, 2022. [cited by applicant]
Examination Report as received in AU application 2022349541 dated Nov. 9, 2023. [cited by applicant]
Examination Report as received in CA application 3,219,050 dated Nov. 24, 2023. [cited by applicant]
U.S. Appl. No. 17/481,176, filed Jan. 12, 2022, Office Action. [cited by applicant]
U.S. Appl. No. 17/481,176, filed Mar. 15, 2022, Notice of Allowance. [cited by applicant]
U.S. Appl. No. 17/656,986, filed Jul. 6, 2023, Office Action. [cited by applicant]
U.S. Appl. No. 17/656,986, filed Oct. 5, 2023, Notice of Allowance. [cited by applicant]
Notice of Allowability as received in IL application 314595 dated Sep. 23, 2024. [cited by applicant]
Office Action as received in JP Application 2023-570447 dated Sep. 17, 2024. [cited by applicant]
Final Rejection as received in JP Application 2023-570447 dated Feb. 28, 2025. [cited by applicant]
Written Opinion and Search Report as received in SG application 11202307384P dated Feb. 3, 2025. [cited by applicant]