Methods and systems for optimal capture of a multi-channel image from an LSPR spectrometer
Methods and systems for optimal capture of a multi-channel image in a LSPR spectrometry is described herein. The method comprises 1) finding a plurality of valid groupings of channels, 2) determining total capture times for each valid grouping of channels, 3) determining a measure of exposure sub-optimality, 4) estimating the expected error in peak wavelength for each valid grouping, 5) finding an optimal grouping of channels by identifying the grouping with the lowest estimate of expected error in peak wavelength and 6) capturing a subsequent multi-channel image using the optimal grouping.
1. A method for optimal capture of a multi-channel image in a Localized Surface Plasmon Resonance (LSPR) spectrometer system, the method comprising:
(a) finding one or more groupings of channels using a first image;
(b) determining a total capture time for each of the one or more groupings of channels;
(c) determining a exposure sub-optimality for each channel of the one or more groupings of channels;
(d) estimating an expected error in peak wavelength for each channel of the one or more groupings of channels using the total capture time and the exposure sub-optimality;
(e) finding an optimal grouping of the one or more groupings of channels by identifying a grouping with the lowest estimate of expected error in peak wavelength; and
(f) capturing a subsequent image using the optimal grouping.
2. The method of claim 1 , wherein the grouping of one or more groupings of channels comprises at least 2 channels.
3. The method of claim 1 , wherein the one or more groupings of channels comprises all possible groupings of channels.
4. The method of claim 1 , wherein each of the one or more groupings of channels only comprises contiguous channels.
5. The method of claim 1 , wherein the one or more groupings of channels is encoded using binary numbers.
6. The method of claim 1 , wherein the exposure sub-optimally for a each of the one or more groupings of channels is equal to the lowest exposure sub-optimally across all channels in the grouping.
7. The method of claim 1 , wherein the error in peak wavelength for a is estimated using interpolation of known peak wavelength errors at total frame capture times and exposure sub-optimalities.
8. The method of claim 1 , wherein the error in peak wavelength is estimated using polynomial regression using pre-computed peak wavelength errors at predetermined total frame capture times and exposure sub-optimalities.
9. The method of claim 1 , wherein the exposure sub-optimality for a a grouping of the one or more groupings of channels is equal to the average exposure sub-optimality over all channels in the grouping.
10. The method of claim 1 , wherein the exposure sub-optimality for a a grouping of the one or more groupings of channels is equal to the median exposure sub-optimality across all channels in the grouping.