IP Library › Granted Patent US 12,545,920
Granted Patent B2
US 12,545,920 · App. 18/624,980 · Granted Feb 10, 2026

Increasing gene editing and site-directed integration events utilizing meiotic and germline promoters

Inventors: Matthew J. Bauer (Manchester, MO); Lisa Kanizay (Chesterfield, MO); Jonathan Lamb (Wildwood, MO); Matthew S. Marengo (Wildwood, MO); Brent O'Brien (St. Charles, MO)
Assignee: MONSANTO TECHNOLOGY LLC
C12N15/8213C12N9/22C12N15/11C12N2310/20
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Quick Facts
Patent No.
US 12,545,920
App. No.
18/624,980
Granted
Feb 10, 2026
Kind
B2
Abstract

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

Claims (8)

1 . A recombinant DNA construct comprising

(a) a first nucleic acid sequence encoding a CRISPR effector protein operably linked to 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 transcription activator-like effector (TALE) binding sites and a minimal promoter; and

(b) a second nucleic acid sequence encoding a TALE operably linked to an egg cell-preferred promoter, wherein the egg cell-preferred promoter is an ES4 promoter that comprises a nucleic acid sequence having at least 90% identity to the sequence of SEQ ID NO: 3,

wherein the minimal promoter does not drive expression of the CRISPR effector protein in the absence of TALE binding to one or more of the TALE binding sites.

2 . The recombinant DNA construct of claim 1 , further comprising a third nucleic acid sequence encoding a guide nucleic acid operably linked to a third promoter.

3 . The recombinant DNA construct of claim 1 , wherein the CRISPR effector protein is selected from the group consisting of Cas9, Cas12a, Cas12b and CasX.

4 . The recombinant DNA construct of claim 1 , wherein the minimal promoter is a 35S(-46) promoter.

5 . A plant comprising in its genome the recombinant DNA construct of claim 1 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 4, 2024
From: BAUER, MATTHEW J.; KANIZAY, LISA; LAMB, JONATHAN; MARENGO, MATTHEW S.; O'BRIEN, BRENT
To: MONSANTO TECHNOLOGY LLC
Reel/Frame 067005/0765 →
Continuity (3)
Division 17470784 · Sep 9, 2021
Provisional Application 63076705 · Sep 10, 2020
Related Publication 20240368610A1 · Nov 7, 2024
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