Granted Patent
B2
US 12,516,375 · App. 18/637,154 · Granted Jan 6, 2026
Compositions, devices, systems, and methods for using a nanopore
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Abstract
Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
Claims (18)
1 . A device comprising:
a. a nanopore array comprising two or more nanopores, each nanopore disposed in a membrane;
b. the nanopore array further comprising a sensor circuit in proximity to each nanopore; and
c. a cavity in fluid communication with the two or more nanopores, the cavity substantially filled with a conductive liquid;
wherein the conductive liquid shields the nanopore device from radiation.
2 . The device of claim 1 , wherein the cavity is above the sensor circuit.
3 . The device of claim 1 , wherein the cavity is further coupled to an opening to allow a biological sample to be introduced into the cavity.
4 . The device of claim 1 , wherein the conductive liquid comprises an electrolyte.
5 . The device of claim 1 , further comprising a layer of oxide insulating the conductive liquid from a portion of the nanopore array.
6 . A method for shielding a nanopore device from radiation, comprising:
providing a nanopore array comprising two or more nanopores, each nanopore disposed in a membrane;
the nanopore array further comprising a sensor circuit in proximity to each nanopore; and
providing a cavity in fluid communication with the two or more nanopores, the cavity substantially filled with a conductive liquid;
wherein the conductive liquid shields the nanopore array from radiation.
7 . The method of claim 6 , wherein the cavity is above the sensor circuit.
8 . The method of claim 6 , further providing an opening coupled to the cavity, wherein the opening allows a biological sample to be introduced into the cavity.
9 . The method of claim 6 , wherein the conductive liquid comprises an electrolyte.
10 . The method of claim 6 , further providing a layer of oxide insulating the conductive liquid from a portion of the nanopore device.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 15, 2024
From: AKESON, MARK A.
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 067425/0733 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 15, 2024
From: CHEN, ROGER JINTEH ARRIGO
To: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Reel/Frame 067425/0824 →
Continuity (12)
Provisional Application
61062391
· Jan 25, 2008
Provisional Application
60967539
· Sep 4, 2007
Provisional Application
60962530
· Jul 30, 2007
Provisional Application
60931115
· May 21, 2007
Provisional Application
60921787
· Apr 4, 2007
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