Ganoderma lucidum Designated as Strains M2-100-02 and M2-102-02
Exemplary embodiments include Ganoderma lucidum fungus designated as strains M2-100-02 and M2-102-02 as deposited with the ATCC Patent Depository under the Budapest Treaty (10801 University Blvd, Manassas, VA 20110), on Jul. 28, 2023, with the unofficial ATCC Patent Deposit Designation No. PTA-127609 (strain M2-100-02) and the unofficial ATCC Patent Deposit Designation No. PTA-127610 (strain M2-102-02), and the official deposit date of Jul. 28, 2023 and the official Patent Deposit Designation No PTA-127609 (strain M2-100-02) and the official Patent Deposit Designation No. PTA-127610 (strain M2-102-02). In some embodiments, the improved Ganoderma lucidum fungus designated as strains M2-100-02 and M2-102-02 are characterized by having optimized concentrations of bioactive compounds including Ganoderic acids and triterpines. In some embodiments, a Beta-glucan concentration is 20 percent or greater. In some embodiments, Ganoderma lucidum fungus designated as strains M2-100-02 and M2-102-02 are improved to be grown on hydrated hulled oats.
1 . A Ganoderma lucidum fungus designated as strain M2-100-02 with an unofficial ATCC Patent Deposit Designation No. PTA-127609 (strain M2-100-02) and an official Patent Deposit Designation No. PTA-127609.
2 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a distributed mass of fruitbodies on top and sides of a substrate.
3 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having fruiting initiation between eighteen to twenty days after inoculation.
4 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype optimized to grow on oats.
5 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 35%.
6 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 25% to 35%.
7 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 30%.
8 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 25% to 30%.
9 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 20% to 25%.
10 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , characterized by a phenotype having a beta-glucan content of 25%.
11 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , in a powder form.
12 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , in a capsule form.
13 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , in a hot beverage form.
14 . The Ganoderma lucidum fungus designated as strain M2-100-02 of claim 1 , further comprising various concentrations of Ganoderic acids and triterpines.
15 . A method for propagating a Ganoderma lucidum fungus designated as strain M2-100-02, using a liquid spawn, the method comprising:
culturing a mother culture;
culturing a production culture, the production culture being a sub-culture of the mother culture;
creating a liquid master inoculum using the production culture;
diluting the liquid master inoculum to create a diluted liquid master inoculum;
creating a biomass product using the diluted liquid master inoculum;
slicing the biomass product for dehydration to create a sliced biomass product;
dehydrating the sliced biomass product to create a dehydrated sliced biomass product;
milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product; and
screening the designated particle size of the dehydrated sliced biomass product.
16 . A method for propagating a Ganoderma lucidum fungus designated as strain M2-100-02 using a grain spawn, the method comprising:
culturing a mother culture;
culturing a production culture, the production culture being a sub-culture of the mother culture;
creating a master spawn using the production culture;
creating a production spawn using the master spawn;
creating a biomass product using the production spawn;
slicing the biomass product for dehydration to create a sliced biomass product;
dehydrating the sliced biomass product to create a dehydrated sliced biomass product;
milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product; and
screening the designated particle size of the dehydrated sliced biomass product.
17 . A Ganoderma lucidum fungus designated as strain M2-102-02 with an unofficial ATCC Patent Deposit Designation No. PTA-127610 (strain M2-102-02) and an official Patent Deposit Designation No. PTA-127610.
18 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a distributed mass of fruitbodies on top and sides of a substrate.
19 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having fruiting initiation between eighteen to twenty days after inoculation.
20 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype optimized to grow on oats.
21 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 20% to 35%.
22 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 25% to 35%.
23 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 20% to 30%.
24 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 25% to 30%.
25 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 20% to 25%.
26 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , characterized by a phenotype having a beta-glucan content of 25%.
27 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , in a powder form.
28 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , in a capsule form.
29 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , in a hot beverage form.
30 . The Ganoderma lucidum fungus designated as strain M2-102-02 of claim 17 , further comprising various concentrations of Ganoderic acids and triterpines.
31 . A method for propagating a Ganoderma lucidum fungus designated as strain M2-102-02, using a liquid spawn, the method comprising:
culturing a mother culture;
culturing a production culture, the production culture being a sub-culture of the mother culture;
creating a liquid master inoculum using the production culture;
diluting the liquid master inoculum to create a diluted liquid master inoculum;
creating a biomass product using the diluted liquid master inoculum;
slicing the biomass product for dehydration to create a sliced biomass product;
dehydrating the sliced biomass product to create a dehydrated sliced biomass product;
milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product; and
screening the designated particle size of the dehydrated sliced biomass product.
32 . A method for propagating a Ganoderma lucidum fungus designated as strain M2-102-02 for a grain spawn, the method comprising:
culturing a mother culture;
culturing a production culture, the production culture being a sub-culture of the mother culture;
creating a master spawn using the production culture;
creating a production spawn using the master spawn;
creating a biomass product using the production spawn;
slicing the biomass product for dehydration to create a sliced biomass product;
dehydrating the sliced biomass product to create a dehydrated sliced biomass product;
milling the dehydrated sliced biomass product to select a designated particle size of the dehydrated sliced biomass product; and
screening the designated particle size of the dehydrated sliced biomass product.