METHODS AND COMPOSITIONS FOR TARGET DETECTION
Provided herein are methods and compositions for the detection and identification of targets in a sample, using target-specific guide nucleic acids and nucleic acid-guided nuclease system proteins.
1 - 121 . (canceled)
122 . A method of identifying a target nucleic acid in a sample comprising:
a. providing a plurality of guide nucleic acid (gNA)-nucleic acid-guided nuclease system protein complexes, wherein the complexes are targeted to at least one target nucleic acid, wherein the gNA-nucleic acid-guided nuclease system protein complexes are attached to a substrate, wherein the substrate is silica, plastic, glass, metal, silicon, silica-based materials, functionalized polystyrene, functionalized polyethylene glycol, functionalized organic polymers, nitrocellulose or nylon membranes, paper, or cotton;
b. contacting nucleic acids from a sample with the plurality of gNA-nucleic acid-guided nuclease system protein complexes, wherein the nucleic acids are labeled with an intercalating dye, and wherein the nucleic acids are sheared prior to step (b);
c. washing the substrate to remove the unbound nucleic acids; and
d. identifying the target nucleic acid in the sample;
wherein the identifying is achieved by detecting a signal produced by the intercalating dye on the substrate, wherein the presence of the signal indicates binding of the gNA-nucleic acid-guided nuclease system protein complex to the target nucleic acid.
123 . The method of claim 122 , wherein the nucleic acids from the sample are labeled prior to the contacting step.
124 . The method of claim 122 , wherein the nucleic acids from the sample are labeled after the contacting step.
125 . The method of claim 122 , wherein the nucleic acid-guided nuclease system protein is a CRISPR/Cas system protein.
126 . The method of claim 125 , wherein the CRISPR/Cas system protein is selected from the group consisting of Cas9, CasX, CasY, Cpf1, Cas3, Cas8a-c, Cas10, Csel, Csyl, Csn2, Cas4, Csm2, and Cm5.
127 . The method of claim 126 , wherein the nucleic acid-guided nuclease system protein is a dead nucleic acid-guided nuclease system protein.
128 . The method of claim 127 , wherein the nucleic acid-guided nuclease system protein is dCas 9 .
129 . The method of claim 126 , wherein the nucleic acid-guided nuclease system protein is a nucleic acid-guided nuclease system nickase protein.
130 . The method of claim 122 , wherein the sample is selected from the group consisting of a clinical sample, a forensic sample, an environmental sample, a metagenomic sample, and a food sample.
131 . The method of claim 122 , wherein the sample is from a human.
132 . The method of claim 122 , wherein the complexes are targeted to a plurality of target nucleic acids.
133 . The method of claim 122 , wherein the target nucleic acid is indicative of an individual.
134 . The method of claim 122 , wherein the target nucleic acid is indicative of a pathogen.
135 . The method of claim 122 , wherein the gNA-nucleic acid-guided nuclease system protein complexes comprise at least 1 unique gNA.
136 . The method of claim 122 , wherein the nucleic acid is amplified.
137 . The method of claim 122 , wherein the nucleic acid is blocked at one or more termini with non-extendible nucleotides.
138 . The method of claim 122 , wherein a plurality of targets are simultaneously identified.
139 . The method of claim 138 , wherein the sample is subject to multi-detection steps.
140 . The method of claim 122 , wherein the method is carried out in less than 24 hours.
141 . The method of claim 122 , wherein the nucleic acid comprises or consists of DNA.
142 . The method of claim 122 , wherein the gNA-nucleic acid-guided nuclease system protein complexes are attached to a substrate in a referenceable order.
143 . The method of claim 122 , wherein the nucleic-acid guided nuclease is attached to the substrate.
144 . The method of claim 143 , wherein the nucleic acid-guided nuclease is a Cas9 nuclease.
145 . The method of claim 122 , wherein the complexes are targeted to at least 10 targets and wherein identifying comprises identifying the at least 10 targets in the sample.
146 . A method of identifying a target nucleic acid in a sample comprising:
a. contacting nucleic acid from a sample with a plurality of guide nucleic acid (gNA)-nucleic acid-guided nuclease system protein complexes attached to a substrate, wherein the substrate is silica, plastic, glass, metal, silicon, silica-based materials, functionalized polystyrene, functionalized polyethylene glycol, functionalized organic polymers, nitrocellulose or nylon membranes, paper, or cotton, wherein the complexes are targeted to at least one target nucleic acid, wherein the nucleic acid from the sample comprises a first intercalating dye label and the gNA-nucleic acid-guided nuclease system protein complexes comprise a second label, and wherein the nucleic acid is sheared prior to use in step (a); and
b. identifying the target nucleic acid in the sample, wherein the identifying is achieved by detecting co-localization of the first label and the second label or by detecting a specific signal from the first label and the second label, wherein the co-localization of the first label and the second label or the presence of the specific signal indicates binding of the gNA-nucleic acid-guided nuclease system protein complex to the target nucleic acid.
147 . The method of claim 146 , wherein the nucleic acid and the plurality of the gNA-nucleic acid-guided nuclease system protein complexes are both labeled prior to the contacting step.
148 . The method of claim 146 , wherein the nucleic acid is labeled after contacting, and the plurality of the gNA-nucleic acid-guided nuclease system protein complexes are labeled before the contacting step.
149 . The method of claim 146 , wherein the first and second label comprise a donor/acceptor pair for fluorescent resonance energy transfer.
150 . The method of claim 146 , wherein the identifying comprises detecting multiple signals.
151 . The method of claim 150 , wherein the nucleic acid, the gNA-nucleic acid-guided nuclease system protein complexes, or both the nucleic acid and the gNA-nucleic acid-guided nuclease system protein complexes comprise multiple labels.
152 . The method of claim 146 , wherein the gNA-nucleic acid-guided nuclease system protein complexes are attached to a substrate in a referenceable order.
153 . The method of claim 146 , wherein the nucleic-acid guided nuclease is attached to the substrate.
154 . The method of claim 153 , wherein the nucleic acid-guided nuclease is a Cas9 nuclease.
155 . The method of claim 146 , wherein the complexes are targeted to at least 10 targets and wherein identifying comprises identifying the at least 10 targets in the sample.