Delivery of therapeutics in vivo via a crispr-based cascade system
The present disclosure relates to compositions of matter and methods used to activate effector nucleic acids and effector targets in vivo via a CRISPR-based cascade system. The compositions and methods achieve non-specific delivery of cascade system components to cells yet the cascade system works in a cell-specific manner.
1 . A method for activating an effector nucleic acid in vivo in a cell comprising the steps of:
providing cascade system components, wherein the cascade system components comprise:
a plurality of combination guide nucleic acids, wherein each combination guide nucleic acid comprises 1) a first guide nucleic acid portion comprising a region that binds to a first nucleic acid-guided nuclease to form a first ribonucleoprotein complex and a region complementary to a target nucleic acid of interest coupled by a linker to 2) a blocked second guide nucleic acid portion comprising a) a region that binds to a second nucleic acid-guided nuclease, b) a region complementary to an effector nucleic acid, c) regions of complementarity to the region complementary to the effector nucleic acid forming clamp sequences, and d) regions of non-complementarity to the region complementary to the effector nucleic acid, wherein at least one of the regions of non-complementarity forms at least one loop, and wherein the blocked second guide nucleic acid portion initially is blocked and unable to bind the effector nucleic acid or form a second ribonucleoprotein complex with the second nucleic acid-guided nuclease; and
the first and second nucleic acid-guided nucleases, wherein the first and second nucleic acid-guided nucleases are nucleic acid targeting nucleic acid-guided nucleases with cis-cleavage activity and collateral trans-cleavage activity, and wherein the first and second nucleic acid-guided nucleases are selected from Cas3, Cas12a, Cas12b, Cas12c, Cas12d, Cas12e, Cas14, Cas12h, Cas12i, and Cas12j, Cas13a, Cas13b, and Cas12g;
delivering the cascade system components to cells under conditions that allow the target nucleic acids of interest in the cells, if present, to bind to the first ribonucleoprotein complex, wherein upon binding of the target nucleic acid of interest to the first ribonucleoprotein complex, the first ribonucleoprotein complex becomes active initiating trans-cleavage activity of the first ribonucleoprotein complex thereby cleaving the blocked second guide nucleic acid portion from the combination guide nucleic acid and unblocking the blocked second guide nucleic acid portion producing at least one unblocked second guide nucleic acid, and wherein the at least one unblocked second guide nucleic acid forms a second ribonucleoprotein complex with the second nucleic acid-guided nuclease and is able to bind to and activate the effector nucleic acid in the cell.
2 . The method of claim 1 , wherein the first guide nucleic acid portion of the combination guide nucleic acid is approximately 63 to 70 nucleotides in length.
3 . The method of claim 1 , wherein the linker of the combination guide nucleic acid is approximately 5-50 nucleotides in length.
4 . The method of claim 3 , wherein the linker of the combination guide nucleic acid is approximately 10-40 nucleotides in length.
5 . The method of claim 4 , wherein the linker of the combination guide nucleic acid is approximately 10-20 nucleotides in length.
6 . The method of claim 5 , wherein the linker of the combination guide nucleic acid is single-stranded.
7 . The method of claim 1 , wherein the at least one loop comprises 3-10 nucleotides.
8 . The method of claim 7 , wherein the at least one loop comprises 3 nucleotides.
9 . The method of claim 7 , wherein the at least one loop comprises 5 nucleotides.
10 . The method of claim 7 , wherein the at least one loop comprises 7 nucleotides.
11 . The method of claim 1 , wherein the regions of non-complementarity form two loops.
12 . The method of claim 11 , wherein the two loops each comprise 3-10 nucleotides.
13 . The method of claim 12 , wherein at least one of the two loops comprises 3 nucleotides.
14 . The method of claim 12 , wherein at least one of the two loops comprises 5 nucleotides.
15 . The method of claim 12 , wherein at least one of the two loops comprises 7 nucleotides.
16 . The method of claim 1 , wherein the regions of non-complementarity form three loops.
17 . The method of claim 16 , wherein the three loops each comprise 3-10 nucleotides.
18 . The method of claim 17 , wherein at least one of the three loops comprises 3 nucleotides.
19 . The method of claim 16 , wherein at least one of the three loops comprises 5 nucleotides.
20 . The method of claim 16 , wherein at least one of the three loops comprises 7 nucleotides.
21 . The method of claim 1 , wherein there are two loops and the loops are different sizes.
22 . The method of claim 1 , wherein there are three loops and the loops are all different sizes.
23 . The method of claim 1 , wherein the first guide nucleic acid portion binds to an RNA-guided RNA endonuclease and the blocked second guide nucleic acid portion binds to an RNA-guided DNA endonuclease.
24 . The method of claim 1 , wherein the first guide nucleic acid portion binds to an RNA-guided DNA endonuclease and the blocked second guide nucleic acid portion binds to an RNA-guided RNA endonuclease.
25 . The method of claim 1 , wherein the first guide nucleic acid portion and the blocked second guide nucleic acid portion binds to an RNA-guided RNA endonuclease.
26 . The method of claim 1 , wherein the first guide nucleic acid portion and the blocked second guide nucleic acid portion binds to an RNA-guided DNA endonuclease.
27 . The method of claim 1 , further comprising a hairpin loop formed at the end of a clamp sequence.
28 . The method of claim 1 , comprising at least two different combination guide nucleic acid molecules, wherein different combination guide nucleic acid molecules comprise different first guide nucleic acid portions that detect different target nucleic acids of interest and wherein different combination guide nucleic acid portions comprise different second guide nucleic acid sequences that activate different effector nucleic acids.
29 . The method of claim 1 , comprising at least two different combination guide nucleic acid molecules, wherein different combination guide nucleic acid molecules comprise different first guide nucleic acid portions that detect different target nucleic acids of interest and wherein different combination guide nucleic acid portions comprise the same second guide nucleic acid sequences that activate the same effector nucleic acids.
30 . The method of claim 1 , wherein the effector nucleic acid, when activated, acts upon an effector target.