Granted Patent
B2
US 12,516,112 · App. 18/669,092 · Granted Jan 6, 2026
Single-chain chimeric polypeptides and uses thereof
Assignee:
ImmunityBio, Inc.
C07K16/18A61K39/00A61P3/10C07K16/2809C07K16/2818C07K16/2866C12N5/0636C12N5/0637C07K2317/24C07K2317/56C07K2317/622C07K2319/00C12N2501/998
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Abstract
Provided herein are single-chain chimeric polypeptides that include: (i) a first target-binding domain; (ii) a soluble tissue factor domain; and (iii) a second target-binding domain. Also provided here are methods of using these single-chain chimeric polypeptides and nucleic acids encoding these single-chain chimeric polypeptides.
Claims (34)
1 . A method of treating a subject having hyperglycemia, the method comprising administering to the subject a therapeutically effective amount of a single-chain chimeric polypeptide comprising:
(i) a first target-binding domain comprising a sequence that is at least 80% identical to SEQ ID NO: 28;
(ii) a soluble tissue factor domain comprising a sequence that is at least 80% identical to SEQ ID NO: 9 and does not initiate blood coagulation; and
(iii) a second target-binding domain comprising a sequence that is at least 80% identical to SEQ ID NO: 28;
wherein the first target-binding domain and the second target-binding domain comprise interleukin-2 (IL-2), and
wherein the single-chain chimeric polypeptide does not stimulate blood coagulation in a mammal.
2 . The method of claim 1 , wherein the first target-binding domain and the soluble tissue factor domain directly abut each other.
3 . The method of claim 1 , wherein the single-chain chimeric polypeptide further comprises a linker sequence between the first target-binding domain and the soluble tissue factor domain.
4 . The method of claim 1 , wherein the soluble tissue factor domain and the second target-binding domain directly abut each other.
5 . The method of claim 1 , wherein the single-chain chimeric polypeptide further comprises a linker sequence between the soluble tissue factor domain and the second target-binding domain.
6 . The method of claim 1 , wherein:
the first target-binding domain comprises a sequence that is at least 90% identical to SEQ ID NO: 28;
the soluble tissue factor domain comprises a sequence that is at least 90% identical to SEQ ID NO: 9, and
the second target-binding domain comprises a sequence that is at least 90% identical to SEQ ID NO: 28.
7 . The method of claim 6 , wherein:
the first target-binding domain comprises a sequence that is at least 95% identical to SEQ ID NO: 28;
the soluble tissue factor domain comprises a sequence that is at least 95% identical to SEQ ID NO: 9; and
the second target-binding domain comprises a sequence that is at least 95% identical to SEQ ID NO: 28.
8 . The method of claim 7 , wherein:
the first target-binding domain comprises SEQ ID NO: 28;
the soluble tissue factor domain comprises SEQ ID NO: 9; and
the second target-binding domain comprises SEQ ID NO: 28.
9 . The method of claim 1 , wherein the soluble tissue factor domain is a soluble human tissue factor domain that does not have coagulation activity.
10 . The method of claim 1 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 90% identical to SEQ ID NO: 108.
11 . The method of claim 10 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 95% identical to SEQ ID NO: 108.
12 . The method of claim 11 , wherein the single-chain chimeric polypeptide comprises SEQ ID NO: 108.
13 . The method of claim 1 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 90% identical to SEQ ID NO: 110.
14 . The method of claim 13 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 95% identical to SEQ ID NO: 110.
15 . The method of claim 14 , wherein the single-chain chimeric polypeptide comprises SEQ ID NO: 110.
16 . The method of claim 1 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 80% identical to SEQ ID NO: 108.
17 . The method of claim 1 , wherein the single-chain chimeric polypeptide comprises a sequence that is at least 80% identical to SEQ ID NO: 110.
18 . The method of claim 1 , wherein fasting blood glucose levels are lowered.
19 . The method of claim 1 , wherein insulin resistance is reduced.
20 . The method of claim 1 , wherein the subject has type 2 diabetes mellitus.
Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Jul 17, 2024
From: HCW BIOLOGICS INC.
To: IMMUNITYBIO, INC.
Reel/Frame 068014/0474 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 23, 2024
From: WONG, HING
To: HCW BIOLOGICS, INC.
Reel/Frame 067508/0043 →
Continuity (10)
Provisional Application
62881039
· Jul 31, 2019
Provisional Application
62817241
· Mar 12, 2019
Provisional Application
62817244
· Mar 12, 2019
Provisional Application
62816683
· Mar 11, 2019
Provisional Application
62749506
· Oct 23, 2018
Provisional Application
62746832
· Oct 17, 2018
Provisional Application
62725038
· Aug 30, 2018
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