Anti-Bed Bug Monoclonal Antibodies and Methods of Making and Uses Thereof
The present disclosure provides anti-bed bug monoclonal antibodies and antigen-binding fragments thereof as well as compositions and kits comprising the same. The present disclosure also provides methods of making monoclonal antibodies and antigen-binding fragments thereof and methods of using the same to detect bed bugs.
1 . An antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7], or an antigen-binding fragment thereof.
2 . (canceled)
3 . A monoclonal antibody or an antigen-binding fragment thereof comprising the heavy and light chain complementarity determining regions of the antibody of claim 1 .
4 . The antibody or antigen-binding fragment of claim 3 , comprising the heavy and light chain variable regions of the antibody of claim 1 .
5 . The antibody or antigen-binding fragment of claim 4 , comprising the heavy and light chains of the antibody of claim 1 .
6 . The antibody or antigen-binding fragment of claim 1 , wherein the antibody or antigen-binding fragment is capable of binding to a bed bug antigen in a lysate of whole bed bugs or an extract of collection paper comprising bed bug waste material.
7 . A mutant of the antibody or antigen-binding fragment of claim 1 , wherein the mutant is capable of binding to a bed bug antigen in a lysate of whole bed bugs or an extract of collection paper comprising bed bug waste material.
8 . (canceled)
9 . A conjugated monoclonal antibody or conjugated antigen-binding fragment, comprising the antibody or antigen-binding fragment of claim 1 and a detection agent.
10 . The conjugated antibody or conjugated antigen-binding fragment of claim 9 , wherein the detection agent is colloidal gold.
11 . The conjugated antibody or conjugated antigen-binding fragment of claim 9 , comprising the antibody produced by the hybridoma deposited at the ATCC under Accession Number PTA-122644 [BB2], or an antigen binding fragment thereof.
12 . The conjugated antibody or conjugated antigen-binding fragment of claim 9 , comprising the antibody produced by the hybridoma deposited at the ATCC under Accession Number PTA-122645 [BB7], or an antigen binding fragment thereof.
13 . A composition comprising the antibody or antigen-binding fragment of claim 1 .
14 . A composition comprising:
(a) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], and
(b) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], or the conjugated antibody of claim 12 .
15 . A composition comprising:
(a) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], and
(b) an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], or the conjugated antibody of claim 11 .
16 . A kit comprising the antibody or antigen-binding fragment of claim 1 .
17 . A hybridoma capable of producing an antibody, wherein the hybridoma is deposited at the ATCC under Accession Number PTA-122644 [BB2] or PTA-122645 [BB7].
18 . (canceled)
19 . An isolated cell producing the antibody or antigen-binding fragment of claim 1 .
20 . A method of making the antibody or antigen-binding fragment of claim 1 , comprising (a) culturing a cell that produces the antibody or antigen-binding fragment, and (b) isolating the antibody or antigen-binding fragment from the cultured cell.
21 . A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with the antibody or antigen-binding fragment of claim 1 , and detecting binding of the bed bug antigen to the antibody or antigen-binding fragment.
22 . (canceled)
23 . A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122644 [BB2], the conjugated antibody of claim 12 .
24 . A method of detecting bed bugs, comprising contacting a sample comprising a bed bug antigen with an antibody produced by a hybridoma deposited at the American Type Culture Collection (ATCC) under Accession Number PTA-122645 [BB7], and the conjugated antibody of claim 11 .
25 - 27 . (canceled)