METHODS FOR DETECTION OF MEMBRANE BOUND GLYPICAN-3
Embodiments provide for anti-GPC3 antibodies compositions comprising the same, and methods of using such antibodies and compositions for the prevention, diagnosis, and treatment of cancer. In one embodiment, a method for predicting a therapeutic effect of an anti-GPC3 immunotherapy on a cancer characterized in that cells of the cancer express GPC3, comprises detecting the presence of said cells in a subject via an immunohistochemical methodology, and wherein when the presence of said cells is detected, the anti-GPC3 immunotherapy is predicted to have a therapeutic effect on the cancer in the subject.
1 . An isolated monoclonal antibody that binds glypican-3 (GPC3), wherein:
the heavy chain of the anti-GPC3 antibody comprises a complementary determining region (CDR) 1 set forth as SEQ ID NO: 6, a CDR2 set forth as SEQ ID NO: 8, and a CDR3 set forth as SEQ ID NO: 10, and the light chain of the antibody comprises a CDR1 set forth as SEQ ID NO: 13, a CDR2 set forth as SEQ ID NO: 15, and a CDR3 set forth as SEQ ID NO: 17.
2 . The isolated monoclonal antibody of claim 1 , wherein:
The heavy chain of the antibody comprises a CDR1, a CDR2, and a CDR3, respectively set forth as amino acid residues 31-35, 50-66, and 99-105 of SEQ ID NO: 2, and the light chain of the antibody comprises a CDR1, a CDR2, and a CDR3 respectively set forth as amino acid residues 24-34, 50-56, and 89-97 of SEQ ID NO: 4.
3 . The isolated monoclonal antibody of claim 1 , wherein:
the heavy chain of the antibody comprises SEQ ID NO: 2, and the light chain of the antibody comprises SEQ ID NO: 4; or
the antibody comprises:
(i) a variable heavy (VH) domain comprising the amino acid sequence of SEQ ID NO: 2; and
(ii) a variable light (VL) domain comprising the amino acid sequence of SEQ ID NO: 4.
4 . (canceled)
5 . The isolated monoclonal antibody of claim 1 , wherein:
the antibody is a chimeric, humanized, or human antibody;
the antibody is a bispecific antibody; or
the antibody is an antibody fragment.
6 . (canceled)
7 . (canceled)
8 . The isolated monoclonal antibody of claim 5 , wherein the antibody is a Fab fragment, a Fab′ fragment, a F(ab)′ 2 fragment, a single chain variable fragment (scFv), or a disulfide stabilized variable fragment (dsFv).
9 . A method of detecting GPC3 in a tissue preparation, the method comprising:
contacting the tissue preparation with the isolated monoclonal antibody of claim 1 under conditions sufficient for formation of a complex of the isolated monoclonal antibody of claim 1 with GPC3 present on the cell membrane of cells of the tissue preparation; and
detecting binding of the antibody to the tissue preparation.
10 . The method of claim 9 , wherein the tissue preparation comprises a hepatocellular carcinoma (HCC), melanoma, squamous cell carcinoma of the lung, Merkel cell carcinoma, or ovarian clear cell carcinoma tumor biopsy.
11 . The method of claim 9 , wherein the monoclonal antibody is directly labeled.
12 . The method of claim 9 , further comprising:
contacting a second antibody that specifically binds the monoclonal antibody with the tissue preparation; and
detecting the binding of the second antibody.
13 . The method of claim 9 , wherein detecting the binding of the antibody to the tissue sample further comprises scoring an amount of the complex detected.
14 . The method of claim 13 , wherein said scoring is done by a pathologist.
15 . The method of claim 13 , wherein said detecting the presence of the complex is done via digitization; and
wherein said scoring is automated based on the digitization of the detected complex.
16 . The method of claim 13 , wherein said scoring further comprises determining a staining intensity of the complex detected via immunohistochemistry using an integer scale from 0 (negative) to 3+, recording the percentage of positively stained cells at each intensity level, and calculating a membrane-associated H-score based on the percentage of positively stained cells at each intensity level.
17 . A method for predicting a therapeutic effect of an anti-GPC3 immunotherapy on a cancer, the cancer characterized in that cells of the cancer express GPC3, the method comprising:
detecting the presence of said cells in a subject via the method of claim 9 , wherein when the complex of the anti-GPC3 antibody with GPC3 expressed on the membrane of the cancer cells is detected, the anti-GPC3 immunotherapy is predicted to have a therapeutic effect on the cancer in the subject.
18 . The method of claim 17 , wherein the method of predicting the therapeutic effect is conducted prior to the subject having received any anti-GPC3 immunotherapy.
19 . The method of claim 17 , wherein the method of predicting the therapeutic effect is conducted while the subject is already in the process of receiving the anti-GPC3 immunotherapy.
20 . The method of claim 17 , wherein the anti-GPC3 immunotherapy comprises an anti-GPC3 antibody, or a chimeric antigen receptor (CAR) T cell therapy, or CAR NK cell therapy, wherein the CAR is designed to specifically recognize membrane-bound GPC3.
21 . (canceled)
22 . An isolated nucleic acid molecule encoding the monoclonal antibody of claim 1 .
23 . The isolated nucleic acid molecule of claim 22 , wherein:
a nucleotide sequence encoding the heavy chain of the monoclonal antibody comprises SEQ ID NO: 1 and a nucleotide sequence encoding the light chain of the antibody comprises SEQ ID NO: 3.
24 . An expression vector comprising the isolated nucleic acid molecule of claim 22 , or a host cell transformed with the expression vector.
25 . (canceled)
26 . (canceled)
27 . An antibody-drug conjugate (ADC), comprising the isolated monoclonal antibody of claim 1 .
28 . A chimeric antigen receptor (CAR) comprising the antibody fragment of claim 7 .
29 . A modified immune cell, comprising a chimeric antigen receptor (CAR), wherein said CAR comprises the CAR of claim 28 , or a plurality of the modified immune cells.
30 . The modified immune cell of claim 29 , wherein the modified immune cell is a modified T cell or a modified NK cell.
31 . The modified immune cell of claim 30 , wherein the modified immune cell is an αβ T cell, or a γδ T cell.
32 . (canceled)
33 . (canceled)
34 . (canceled)
35 . A method of inhibiting the growth of a cell that displays a GPC3 epitope that is specifically recognized by the antibody of claim 1 , comprising contacting said cell with the isolated monoclonal antibody of claim 1 .
36 . A composition comprising a therapeutically effective amount of the isolated monoclonal antibody of claim 1 , and a pharmaceutically acceptable carrier.
37 . A method of treating a subject having a cancer, comprising selecting a subject with a cancer that expresses GPC3 and administering to said subject the composition according to claim 35 , thereby treating the cancer in the subject.
38 . The method of claim 37 , wherein the cancer is liver cancer, ovarian cancer, gastric cancer, Merkel cell carcinoma, or lung cancer.
39 . (canceled)
40 . (canceled)
41 . (canceled)
42 . (canceled)