COATED SUBSTRATES WITH EXTENDED SHELF LIFE
The present disclosure relates to coated substrates, capillaries and microfluidic channels which exhibit an extended shelf life compared to uncoated substrates. More specifically the disclosure relates to charged coatings at physiological pH which may prolong the shelf life of the substrate, capillary or microfluidic channel before being applied to biological fluids. Said shelf life is measured by the ability to provide a low fluid contact angle after an extended storage interval in ambient atmospheric conditions.
1 . A coated substrate comprising:
a substrate, said substrate comprising one or more of glass, metal, ceramic and polymer;
a coating, said coating comprising one or more coating layers, wherein the one or more coating layers coat at least one surface of the substrate, and wherein at least one of said one or more coating layers independently comprises carboxylate function, amine function, polycarboxylate function, polyamine function or combinations thereof.
2 . The coated substrate according to claim 1 , wherein the contact angle of a biological fluid on the coated surface is less than 50°, or less than 40°, or less than 30°, or less than 25°.
3 . The coated substrate according to claim 1 , wherein the contact angle of a biological fluid on the coated surface does not increase by more than 30° after 6 months storage under ambient conditions of temperature and pressure.
4 . The coated substrate according to claim 2 , wherein the biological fluid comprises an interstitial fluid, saliva, urine, tear fluid, mucus or blood.
5 - 7 . (canceled)
8 . The coated substrate according to claim 1 , wherein at least two of said one or more coating layers comprise carboxylate function, amine function, polycarboxylate function, polyamine function or combinations thereof.
9 . The coated substrate according to claim 1 , wherein the coating layers comprise one or more polyelectrolytes and/or one or more poly(amino acids).
10 . The coated substrate according to claim 9 , wherein the coating layers comprise one or more anionic polyelectrolytes and one or more cationic polyelectrolytes.
11 - 12 . (canceled)
13 . The coated substrate according to claim 9 , wherein at least one of the coating layers comprises poly(allylamine).
14 . The coated substrate according to claim 10 , wherein the coating layers comprises one or more anionic poly(amino acids) and one or more cationic poly(amino acids).
15 - 18 . (canceled)
19 . The coated substrate according to claim 1 , wherein the coating layers comprise n+2 layers, comprising
a first layer and when present all odd number layers comprising anionic poly(electrolyte) or an anionic poly(amino acid); and
a second layer and when present all even number layers comprising cationic poly(electrolyte) or a cationic poly(amino acid),
wherein the first layer is in contact with the substrate; and
wherein n is an integer between 0 and 300.
20 . The coated substrate according to claim 1 , wherein the coating layers comprise n+2 layers, comprising
a first layer and when present all odd number layers comprising cationic poly(electrolyte) or cationic poly(amino acid); and
a second layer and when present all even number layers comprising anionic poly(electrolyte) or anionic poly(amino acid),
wherein the first layer is in contact with the substrate; and
wherein n is an integer between 0 and 300.
21 - 22 . (canceled)
23 . The coated substrate according to claim 19 , wherein n is an integer between 2 and 100 or between 2 and 20 or n is at least 10.
24 - 25 . (canceled)
26 . The coated substrate according to claim 1 , wherein the glass comprises borosilicate glass, and wherein the ratio of the percentage of metal atoms to silicon and boron atoms in the borosilicate glass is at least 0.1.
27 . The coated substrate according to claim 1 , wherein the thickness of the coating is from about 1 nm to about 450 nm, or from about 3 nm to about 100 nm, or from about 3 nm to about 30 nm.
28 . (canceled)
29 . The coated substrate according to claim 1 , wherein one or more layers of the coated substrate is a spray coated layer.
30 . The coated substrate according to claim 1 , wherein the substrate comprises a capillary tube or a microfluidic channel.
31 . The capillary tube or microfluidic channel according to claim 30 , wherein the capillary tube or microfluidic channel comprises glass.
32 . The capillary tube or microfluidic channel according to claim 30 , wherein the coating coats an inner surface of the capillary tube or microfluidic channel.
33 . The capillary tube or microfluidic channel according to claim 32 , wherein the capillary tube or microfluidic channel has an aspect ratio (length: inner diameter) of from about 2:1 to about 150:1 and/or wherein the capillary tube has an aspect ratio (length: cross sectional area) of from about 2:1 to about 191:1.
34 . (canceled)
35 . The capillary tube or microfluidic channel according claim 32 , wherein the capillary tube or microfluidic channel has a minimum internal diameter of about 358 micrometres and a maximum internal diameter of about 2000 micrometres and/or wherein the capillary or channel has a minimum cross sectional area of about 0.1 mm 2 and a maximum cross sectional area of about 3.1 mm 2 .
36 . (canceled)
37 . The capillary tube or microfluidic channel according to claim 32 , wherein the capillary tube fills with blood to a predetermined level when held in the vertical orientation to the blood sample and wherein the predetermined level is greater than 90%, preferably greater than or equal to 95% of the capillary volume under ambient conditions.
38 . The capillary tube according to claim 37 , wherein the capillary fills to the predetermined level in less than about 12 seconds, preferably less than about 11 seconds.
39 . The capillary tube according to claim 32 , wherein the capillary tube is stored for at least 6 months or at least 9 months, or at least 12 months at ambient conditions of temperature and pressure prior to filling with blood.
40 - 46 . (canceled)
47 . The coated substrate according to claim 1 wherein the coating layers comprise an outer layer comprising anionic polyelectrolyte or anionic poly(amino acid).
48 . The coated substrate according to claim 9 , wherein the outer layer comprises poly(acrylic acid), poly(arginine), poly(glutamic acid) or poly(lysine).
49 . The coated substrate according to claim 20 , wherein n is an integer between 2 and 100 or between 2 and 20 or n is at least 10.