DIGITAL MICROFLUIDICS (DMF) SYSTEM, INSTRUMENT, AND CARTRIDGE INCLUDING MULTI-SIDED DMF DISPENSING AND METHOD

1 . A method, the method comprising:

loading a multi outlet digital microfluidic device dispenser with a liquid, the multi outlet digital microfluidic device dispenser comprising a first portion having a height that is different from a second portion of the multi outlet digital microfluidic device;

dispensing a first portion of the liquid from a first outlet;

dispensing a second portion of the liquid from a second outlet;

performing a first analysis on the first portion of the liquid;

performing a second analysis on the second portion of the liquid; and

presenting results of the first analysis and/or the second analysis.

2 . The method of claim 1 , wherein the first portion of the liquid is roughly the same amount as the second portion of the liquid.

3 . (canceled)

4 . The method of claim 1 , wherein the method further comprises presenting results of the second analysis.

5 . The method of claim 1 , wherein the first analysis and the second analysis are the same analysis.

6 . The method of claim 1 , wherein the method further comprises dispensing the first portion of the liquid from a first outlet includes dispensing the first portion of the liquid to a first track.

7 . The method of claim 6 , wherein dispensing the second portion of the liquid from a second outlet includes dispensing the second portion of the liquid to a second track.

8 . A system, the system comprising:

a multi outlet digital microfluidic device dispenser the multi outlet digital microfluidic device dispenser comprising a first portion having a height that is different from a second portion of the multi outlet digital microfluidic device;

a graphical user interface; and

a processor configured to:

load the multi outlet digital microfluidic device dispenser with a liquid;

dispense a first portion of the liquid from a first outlet;

dispense a second portion of the liquid from a second outlet;

perform a first analysis on the first portion of the liquid;

perform a second analysis on the second portion of the liquid; and

present results of the first analysis and/or second analysis on the graphical user interface.

9 . The system of claim 8 , wherein the first portion of the liquid is roughly the same amount as the second portion of the liquid.

10 . (canceled)

11 . The system of claim 8 , the processor further configured to present results of the second analysis.

12 . The system of claim 8 , wherein the first analysis and the second analysis are the same analysis.

13 . The system of claim 8 , wherein dispensing the first portion of the liquid from a first outlet includes dispensing the first portion of the liquid to a first track.

14 . The system of claim 13 , wherein dispensing the second portion of the liquid from a second outlet includes dispensing the second portion of the liquid to a second track.

15 .- 21 . (canceled)

22 . A microfluidic cartridge, the cartridge comprising:

a top substrate;

a bottom substrate, the bottom substrate having a plurality of droplet operations electrodes, the bottom substrate and top substrate separated by a droplet operations gap therebetween; and

a plurality of multi-outlet dispensers operable to dispense a liquid; and

wherein said droplet operations electrodes include a plurality of dispending electrodes leading away from each of the plurality of multi-outlet dispensers;

the microfluidic cartridge comprising a first portion having a height that is different from a second portion of the microfluidic cartridge.

23 . The microfluidic cartridge of claim 22 , wherein the plurality of multi-outlet dispensers includes one or more dual-sided dispensers, wherein the dual-sided dispensers are operable to dispense a liquid from a first outlet and a second outlet.

24 . The microfluidic cartridge of claim 22 , wherein the plurality of multi-outlet dispensers includes one or more multi-sided dispensers, wherein the multi-sided dispensers are operable to dispense a liquid from multiple outlets.

25 . (canceled)

26 . The microfluidic cartridge of any one of claim 22 , wherein the plurality of multi-outlet dispensers are arranged in an array, and wherein the array comprises one or more rows and/or one or more columns of multi-outlet dispensers.

27 . The microfluidic cartridge of claim 22 , wherein the cartridge further comprises one or more reservoirs, wherein the one or more reservoirs are in fluid communication with the plurality of multi-outlet dispensers. droplet operations electrodes

28 . The microfluidic cartridge of claim 22 , wherein the cartridge further comprises one or more detection mechanisms in fluid communication with the plurality of multi-outlet dispensers.

29 . The microfluidic cartridge of claim 27 , wherein the fluid communication comprises one or more of the droplet operations electrodes.

30 . The microfluidic cartridge of claim 22 , wherein each of the plurality of multi-outlet dispensers includes a liquid reagent or a liquid sample, and optionally wherein the liquid reagent is selected from a wash buffer, a ligand-containing reagent, an antigen-containing reagent, or a reactant-containing reagent.