METHOD OF MOUNTING A BIOLOGICAL SAMPLE ON A SURFACE
Methods for mounting or sticking a biological sample on a surface by contacting the biological sample on the surface with a reducing agent.
1 . A method of mounting a biological sample on a surface, which comprises:
i) applying a biological sample to a surface,
ii) contacting said biological sample on said surface with a reducing agent, optionally followed by
iii) contacting said biological sample on said surface with an oxidizing agent.
2 . The method according to claim 1 , wherein said surface comprises glass, plastics, metal, or quartz.
3 . The method according to claim 1 , wherein said surface is flat or round, smooth or rough, hydrophilic or lipophilic.
4 . The method according to claim 1 , wherein said surface is modified or coated to comprise further functionalities.
5 . The method according to claim 1 , wherein said surface is on or is a microscopic slide.
6 . The method according to claim 1 , wherein said biological sample comprises a biomolecule, a cell, a cell culture, a tissue section, an organ, an organoid and/or an organoculture, or an whole organism.
7 . The method according to claim 1 , wherein said biological sample comprises a biomolecule that contains at least a disulfid-bridge, in particular an oxidized thiol-group.
8 . The method according to claim 1 , wherein said biological sample comprises an extract of biomolecules, cells like prokaryotic, archaebacterial or eukaryotic cells, a tissue sample, a tissue section, a whole organ sample, a whole organism, or multiple organisms.
9 . The method according to claim 1 , wherein said biological sample comprises blood, serum, fluid or a tissue like a biopsy sample.
10 . The method according to claim 1 , wherein said biological sample is incubated in a medium or solution before applied to the surface.
11 . The method according to claim 1 , wherein said biological sample is treated by an enzymatic, chemical, or physical treatment or a combination thereof before applied to the surface.
12 . The method according to claim 1 , wherein said biological sample is a tissue sample and the tissue is cut by a microtome or cryotome in tissue sections before applied to the surface.
13 . The method according to claim 1 , wherein said biological sample comprises lung or skin tissue.
14 . The method according to claim 1 , wherein said biological sample comprises a Formalin-Fixed Paraffin-Embedded (FFPE) Tissue.
15 . The method according to claim 14 , wherein the Formalin-Fixed Paraffin-Embedded (FFPE) Tissue is deparaffinized after applied to the surface, in particular by one or more enzymatic treatments, one or more chemical treatments, one or more physical treatments, or a mixture of them.
16 . The method according to claim 1 , wherein said reducing agent is selected from the group consisting of beta-Mercaptoethanol, Dithiothreitol (DTT), Dithioerythrit, sodium borohydride (NaBH 4 ), lithium aluminium hydride (LiAlH 4 ), or another reducing reagent of the galavanic series.
17 . The method according to claim 1 , wherein said oxidizing agent is selected from the group consisting of hydrogen peroxide (H 2 O 2 ), an organic peroxide like tert-butyl hydroperoxide, sodium perborate, potassium permanganate, hypochlorite, or another oxidizing reagent of the galavanic series.
18 - 30 . (canceled)
31 . A kit for mounting a biological sample on a surface, which comprises:
a) a reducing agent, and
b) an oxidizing agent.
32 . The kit according to claim 31 , wherein said reducing agent is selected from the group consisting of beta-Mercaptoethanol, Dithiothreitol (DTT), Dithioerythrit, sodium borohydride (NaBH 4 ), lithium aluminium hydride (LiAlH 4 ), or another reducing reagent of the galavanic series.
33 . The kit according to claim 31 , wherein said oxidizing agent is selected from the group consisting of hydrogen peroxide (H 2 O 2 ), an organic peroxide like tert-butyl hydroperoxide, sodium perborate, potassium permanganate, hypochlorite, or another oxidizing reagent of the galavanic series.