PLANTS WITH REDUCED LIPASE 1 ACTIVITY
The disclosure relates to a series of independent human-induced non-transgenic mutations found at one or more of the Lip1 genes of a plant; plants having these mutations in one or more of their Lip1 genes; and a method of creating and finding similar and/or additional mutations of Lip1 by screening pooled and/or individual plants. The plants disclosed herein exhibit decreased lipase activity without having the inclusion of foreign nucleic acids in their genomes. Additionally, products produced from the plants disclosed herein exhibit increased hydrolytic and oxidative stability and increased shelf life without having the inclusion of foreign nucleic acids in their genomes.
1 . A method for producing a milled plant product, the method comprising: milling grain from a plant that has one or more human induced alterations in a Lip1 gene to produce a milled plant product, wherein the milled plant product has a property selected from the group consisting of: (a) increased hydrolytic stability as compared to a product derived from grain of a wild type plant; (b) increased oxidative stability as compared to a product derived from grain of a wild type plant; (c) reduced triacylglycerides (TAG) to free fatty acid (FFA) production as compared to a product derived from grain of a wild type plant; (d) reduced hexanal production as compared to a product derived from grain of a wild type plant; and (e) one or more of improved color, flavor, texture, aroma, performance or overall sensory preference, as compared to a product derived from grain of a wild type plant.
2 . The method of claim 1 , wherein the plant is a wheat plant having one or more human induced alterations in the Lip1 gene in one or more of the A, B, and D genomes.
3 . The method of claim 2 , wherein the wheat plant has one or more human induced alterations in the Lip1 gene in each of the A and D genomes.
4 . The method of claim 2 , wherein the wheat plant has one or more human induced alterations in the Lip1 gene in each of the A and B genomes.