Granted Patent B2

US 12,570,948 · App. 18/759,602 · Granted Mar 10, 2026

Inventors: Bharat S. Thakkar (Campbell, CA); Matthias Weber (South San Francisco, CA)

Assignee: Cellares Corporation

C12M41/48C12M23/40C12M23/42C12M29/06C12M37/02C12M41/30

Abstract

Claims (31)

1 . A method for sterile fluid transfer between a fluid device and a cartridge for cell processing, the method comprising:

receiving, by an instrument head of a system, the fluid device comprising a sterile liquid transfer port;

aligning the sterile liquid transfer port with one of a plurality of sterile liquid transfer ports of the cartridge using one or more distance sensors of the instrument head to obtain a position of the one of the plurality of sterile liquid transfer ports of the cartridge relative to the instrument head;

connecting the sterile liquid transfer port to the one of the plurality of sterile liquid transfer ports of the cartridge;

engaging a peristaltic pump with a compressible fluidic tubing of a fluid pump module of the fluid device, wherein engaging comprises moving a pneumatic actuator to translate the peristaltic pump relative to the compressible fluidic tubing of the fluid pump module, thereby adjusting an amount of occlusion of the compressible fluidic tubing; and

transferring, via the connected sterile liquid transfer ports, fluid between the fluid device and the cartridge.

2 . The method of claim 1 , further comprising sterilizing internal surfaces of the connected sterile liquid transfer ports prior to transferring the fluid.

3 . The method of claim 1 , further comprising actuating a cap actuator configured to translate caps of each of the connected sterile liquid transfer ports to open a flow path at an interface of the connected sterile liquid transfer ports.

4 . The method of claim 1 , wherein moving the pneumatic actuator is performed until a predetermined pressure of fluid within the compressible fluidic tubing or a predetermined amount of occlusion of the compressible fluidic tubing is achieved.

5 . The method of claim 1 , wherein the aligning is based on data acquired by the one or more distance sensors of the instrument head.

6 . The method of claim 5 , wherein the aligning comprises detecting at least one edge of the one of the plurality of sterile liquid transfer ports of the cartridge using data acquired by the one or more distance sensors.

7 . The method of claim 6 , wherein the detecting the at least one edge comprises detecting a plurality of points on the one of the plurality of sterile liquid transfer ports of the cartridge.

8 . The method of claim 7 , wherein the plurality of points comprises two locations each along three edges of the one of the plurality of sterile liquid transfer ports of the cartridge.

9 . The method of claim 1 , further comprising receiving a cartridge into a docking station of a second portion of the system.

10 . The method of claim 9 , further comprising securing a position of the cartridge within the docking station.

11 . The method of claim 10 , wherein securing the position of the cartridge within the docking station comprises raising a floating platform on which the cartridge is seated to bring the cartridge into contact with a clamping surface of the docking station.

12 . The method of claim 1 , further comprising performing flow fidelity testing prior to transferring the fluid.

13 . The method of claim 1 , further comprising estimating, based on data acquired by one or more sensors of the instrument head, a flow rate of fluid transfer.

14 . The method of claim 13 , wherein the estimating the flow rate of fluid transfer is used to calibrate a volumetric flow rate based on a revolution of a peristaltic pump of the system.

15 . The method of claim 1 , wherein aligning comprises moving the instrument head from an initial position to a final position using data provided by the distance sensor.

16 . A method for fluid transfer between a fluid device and a cartridge for cell processing, the method comprising:

receiving, by an instrument head of a first portion of a system, the fluid device comprising a sterile liquid transfer port;

receiving, by a second portion of the system, the cartridge within a docking station, the cartridge comprising a plurality of sterile liquid transfer ports;

aligning the sterile liquid transfer port with one of a plurality of sterile liquid transfer ports of the cartridge using one or more distance sensors of the instrument head to obtain a position of the one of the plurality of sterile liquid transfer ports of the cartridge relative to the instrument head;

connecting the sterile liquid transfer port of the fluid device to the one of the plurality of sterile liquid transfer ports of the cartridge;

adjusting a position of a pneumatic actuator of the instrument head relative to a fluid pump module of the fluid device, thereby modifying a distance between a peristaltic pump of the instrument head and compressible fluidic tubing of the fluid pump module; and

transferring, via the connected sterile liquid transfer ports and after a predetermined fluid pressure is achieved within the compressible fluidic tubing, fluid between the fluid device and the cartridge.

17 . The method of claim 16 , wherein aligning comprises moving the instrument head from an initial position to a final position using data provided by the distance sensor.

18 . The method of claim 16 , further comprising sterilizing internal surfaces of the connected sterile liquid transfer ports prior to transferring the fluid.

19 . The method of claim 16 , further comprising actuating a cap actuator configured to translate caps of each of the connected sterile liquid transfer ports to open a flow path at an interface of the connected sterile liquid transfer ports.

20 . The method of claim 16 , further comprising securing a position of the cartridge within the docking station, wherein securing the position of the cartridge within the docking station comprises raising a floating platform on which the cartridge is seated to bring the cartridge into contact with a clamping surface of the docking station.

Assignments (2)

Continuity (2)

References Cited (321)