Formulations and methods for skin care
Cosmetic and pharmaceutical formulations comprising specific amino acid combinations are described herein. Such formulations strengthen and enhance skin epithelial cell barrier function, thereby reducing moisture loss from the skin and restoring skin's structural integrity, epithelial differentiation, intercellular adhesion, and intracellular signaling and/or promoting maintenance of these features. Additional formulations described here comprise a therapeutically effective amount of free amino acids and a therapeutically effective amount of a plant extract for skin barrier repair. Methods for administering these formulations for cosmetic and/or therapeutic purposes and uses thereof for same are also described herein, including the use thereof in the preparation of medicaments.
1. A topical formulation, comprising:
(a) as free amino acids, a therapeutically effective amount consisting essentially of a combination of glutamine, glycine, alanine, and serine, or salts thereof,
wherein each free amino acid has a ratio to each of the other free amino acids of: 1:100 or greater; and
(b) a therapeutically effective amount of a plant extract comprising Boswellia extract,
wherein the therapeutically effective amount of the combination and the therapeutically effective amount of the plant extract improve barrier integrity of skin cells when tested by a method detecting EGF expression and determining that EGF is upregulated by a 1.5-40 average fold change of formulation treated as compared to untreated skin,
wherein the therapeutically effective amount of the combination and the therapeutically effective amount of plant extract improve barrier integrity of the skin and/or skin barrier repair.
2. The topical formulation according to claim 1 , wherein each of the free amino acids is present at a concentration of: 0.1 mM or greater; 10 mM or less; or 0.1 mM to 10 mM.
3. The topical formulation according to claim 1 , wherein each of the free amino acids is present at a weight percent to the formulation (wt/wt %) of: 0.001 wt % or greater; 1 wt % or less; or 0.001 wt % to 1 wt %.
4. The topical formulation according to claim 1 , wherein the therapeutically effective amount of the plant extract is present at a weight percentage (w/w %) of the formulation of: 0.025 w/w % or greater; 5 w/w % or less; or 0.025 w/w %-5 w/w %.
5. The topical formulation according to claim 1 , wherein the amino acid combination and the plant extract are in a ratio of: 1:100 or greater; 100:1 or less; or 1:100 to 100:1.
6. The topical formulation according to claim 1 , wherein the plant extract is selected from the group consisting of: Boswellia serrata resin extract, Boswellia serrata extract, olibanum, frankincense, and any combinations thereof.
7. The topical formulation according claim 1 , wherein the therapeutically effective amount of the combination of free amino acids and the therapeutically effective amount of the plant extract inhibit 5-lipoxygenase, decrease mobilization of calcium ions, decrease activation of MAP kinases, or any combination thereof.
8. The topical formulation of claim 1 , further comprising:
a texturing and/or a bulking agent;
a phospholipid;
an anti-caking agent;
a dermatologically acceptable carrier;
additives, or
any combination thereof.
9. The topical formulation of claim 8 , wherein the bulking agent comprises cellulose, microcrystalline cellulose, or combinations thereof.
10. The topical formulation of claim 8 , wherein the phospholipid comprises lecithin, phosphatidylcholine, or combinations thereof.
11. The topical formulation of claim 8 , wherein the anti-caking agent comprises silica.
12. The topical formulation of claim 8 , wherein the additives are selected from the group consisting of: vitamins, minerals, preservatives, desquamation agents, anti-acne agents, anti-wrinkle agents/anti-atrophy agents, hydroxyl acids, anti-oxidants/radical scavengers, chelators, flavonoids, anti-inflammatory agents, anti-cellulite agents, topical anesthetics, tanning agents, skin lightening agents, skin soothing and skin healing agents, antimicrobial and antifungal agents, sunscreen agents, conditioning agents, structuring agents, thickening agents, preservatives and any combinations thereof.
13. The topical formulation according to claim 1 , wherein the free amino acid is in L-form.