IP Library › Patent Application 18777004
Patent Application
App. No. 18/777,004

MICROBIAL INOCULANT COMPOSITIONS AND METHODS

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Patent No.
US None
App. No.
18/777,004
Abstract

A microbial inoculant composition includes aquatic bacterial species. In some embodiments, the microbial inoculant composition includes at least one of an aquatic Pseudomonas spp. and a Clostridium spp.

Claims (30)

1 . A microbial inoculant composition comprising:

a Pseudomonas mandelii;

an aquatic Delftia spp.; and

a Lactobacillus plantarum.

2 . The microbial inoculant composition of claim 1 , further comprising an aquatic Pseudomonas spp.

3 . The microbial inoculant composition of claim 2 , wherein the aquatic Pseudomonas spp. comprises P. moraviensis or P. fluorescens.

4 . The microbial inoculant composition of claim 1 , further comprising a Clostridium spp.

5 . The microbial inoculant composition of claim 4 , wherein the Clostridium spp. comprises Clostridium saccharobutylicum.

6 . The microbial inoculant composition of claim 1 , further comprising a Bacillus spp.

7 . The microbial inoculant composition of claim 6 , wherein the Bacillus spp. comprises B. megaterium, B. subtilis, or B. licheniformis.

8 . The microbial inoculant composition of claim 1 , further comprising an aquatic Chryseobacterium spp.

9 . The microbial inoculant composition of claim 1 , further comprising an aquatic Pseudomonas fluorescens.

10 . The microbial inoculant composition of claim 1 , further comprising Brevundimonas kwangchunensis, Fictibacillus barbaricus/Bacillus barbaricus, a Prosthecobacter spp., Sphingobacterium multivorum, or a Sphingomonas spp.

11 . The microbial inoculant composition of claim 1 , further comprising Bacillus megaterium, Bacillus amyloliquifaciens, Bacillus subtilus, Bacillus pumilus, Sphingosinicella microcystinivorans, Pseudomonas chlororaphis, Pseudomonas mandelii, Pseudomonas umsongensis, Arthrobacter ramosus, Streptomyces yogyakartansis, an Arthrobacter spp., Xanthomonas spp., or Chryseobacterium indologenes.

12 . The microbial inoculant composition of claim 1 , further comprising a yeast strain.

13 . A plant comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the plant.

14 . A seed comprising the microbial inoculant composition of claim 1 adhered to at least a portion of the seed.

15 . A method comprising applying the microbial inoculant composition of claim 1 to a tissue of a plant.

16 . A method comprising applying the microbial inoculant composition of claim 1 to a surface of a seed.

17 . A method comprising applying the microbial inoculant composition of claim 1 to a seed bed.

18 . A method comprising applying the microbial inoculant composition of claim 1 to a field comprising a plurality of plants.

19 . A method of producing a microbial inoculant composition, the method comprising:

providing a Pseudomonas mandelii; an aquatic Delftia spp.; and a Lactobacillus plantarum;

providing culture medium comprising:

a carbon source;

sufficient vinegar to adjust the pH to no higher than 6.8;

iron at a concentration of 1 ppm; and

potassium nitrate;

incubating the Pseudomonas mandelii; the aquatic Delftia spp.; and the Lactobacillus plantarum together in the culture medium under conditions effective to allow aerobic fermentation and anaerobic fermentation.

20 . The method of claim 19 , wherein the Pseudomonas mandelii; the aquatic Delftia spp.; and the Lactobacillus plantarum are incubated at a temperature of at least 15° C.