PORTABLE, LOW-COST PATHOGEN DETECTION AND STRAIN IDENTIFICATION PLATFORM
Methods for detecting the presence of a pathogen infection are described. In particular, this document provides a method of detecting target nucleic acids, such as pathogen-specific RNA, in a biological sample obtained from a subject, where the method comprises using one or more toehold switch sensors and an isothermal amplification step to detect the target nucleic acid. Methods specific for detecting and identify the presence of a virus such as Zika virus are also provided.
1 .- 62 . (canceled)
63 . A composition comprising a sgRNA selected from SEQ ID NOs: 60-649.
64 . The composition of claim 63 , further comprising a Cas nuclease.
65 . The composition of claim 64 , wherein the Cas nuclease is Cas9 or Cas13.
66 . The composition of claim 63 , further comprising a CRISPR targeting RNA (crRNA) and a trans-activating crRNA (tracrRNA).