COMPOSITION AND METHOD FOR GENERATING IMMUNITY TO BORRELIA BURGDORFERI
Provided is an immunogenic composition including a peptide, wherein consecutive amino acids of the peptide include at least amino acids of SEQ ID NO:1 and one or more adjuvant. Also provided is a method of vaccinating a subject against Borrelia burgdorferi, including administering to the subject an effective amount of the immunogenic composition
1 . An immunogenic composition, comprising
a peptide, comprising at least any eight consecutive amino acids from amino acid 186 to amino acid 196 of SEQ ID NO: 1, except that each amino acid corresponding to amino acids 189 and 193 of SEQ ID NO: 1 comprises alanine or is independently substituted with any other amino acid except tyrosine, wherein the peptide further comprises comprises one or both of
(i) amino acids 1-185 of SEQ ID NO: 1, and from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid; and
(ii) amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid; and
one or more adjuvant.
2 . The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1, and from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid.
3 . The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid.
4 . The immunogenic composition of claim 2 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid.
5 . The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid.
6 . The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1.
7 . The immunogenic composition of claim 1 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
8 . The immunogenic composition of claim 5 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
9 . The immunogenic composition of claim 6 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
10 . An immunogenic composition, comprising
an amino acid sequence comprising at least eight consecutive amino acids from amino acid 186 to amino acid 196 of SEQ ID NO: 1,
an adjuvant, and
a means for inhibiting binding of the amino acid sequence to Factor H.
11 . The immunogenic composition of claim 10 , wherein the adjuvant is selected from the group consisting of an aluminum salt, AS04, AS03, monophosphoryl lipid A, poly(I:C), a CpG DNA adjuvant, MF59, an emulsion adjuvant comprising squalene and water, a combination adjuvant comprising block copolymer CRL-8300, squalene, a sorbitan monooleateor, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium salt (DOTAP), 3 β-[N-(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-chol liposome), and a virosomal adjuvant.
12 . The immunogenic composition of claim 10 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1, and from 0 to 2 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid.
13 . The immunogenic composition of claim 10 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid.
14 . The immunogenic composition of claim 12 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 197-218 of SEQ ID NO: 1 is independently substituted with any other amino acid
15 . The immunogenic composition of claim 10 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1, and from 0 to 1 amino acids of amino acids 1-185 of SEQ ID NO: 1 is independently substituted with any other amino acid.
16 . The immunogenic composition of claim 10 , wherein the peptide further comprises amino acids 1-185 of SEQ ID NO: 1.
17 . The immunogenic composition of claim 10 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
18 . The immunogenic composition of claim 15 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
19 . The immunogenic composition of claim 16 , wherein the peptide further comprises amino acids 197-218 of SEQ ID NO: 1.
20 A method of vaccinating a mammalian subject against Borrelia burgdorferi, comprising
administering to the subject the immunogenic composition of claim 1 .