REDUCED GLUTEN GRAINS AND COMPOSITIONS THEREOF
Plants with reduced gluten grains and compositions thereof are disclosed herein.
1 . A wheat plant comprising one or more mutations in a wheat prolamin box binding factor gene (WPBF) in at least one of the A, B, or D genomes, wherein the one or more mutations contributes to grain from said wheat plant having reduced gluten as compared to grain from a wild type plant.
2 . The wheat plant of claim 1 , wherein the one or more mutations is in the DNA binding with one finger (Dof) region of the WPBF gene.
3 . The wheat plant of claim 1 , wherein the one or more mutations in the WPBF gene is in the B and D genomes.
4 . The wheat plant of claim 1 , wherein the one or more mutations in the WPBF gene is in the A and B genomes.
5 . The wheat plant of claim 1 , wherein the one or more mutations in the WPBF gene is in the A and D genomes.
6 . The wheat plant of claim 1 , wherein the one or more mutations in the WPBF gene is in the A, B, and D genomes.
7 . The wheat plant of claim 1 , wherein the one or more mutations results in reduced low molecular weight glutenins in grain from said wheat plant relative to grain from a wild-type wheat plant.
8 . The wheat plant of claim 1 , wherein the one or more mutations results in reduced gliadins in grain from said wheat plant relative to grain from a wild type wheat plant.
9 . The wheat plant of claim 1 , wherein the one or more mutations results in increased or unaltered high molecular weight glutenins in grain from said wheat plant relative to grain from a wild type wheat plant.
10 . The wheat plant of claim 1 , wherein the one or more mutations results in an amino acid change in the WPBF protein selected from the amino acid changes recited in Tables 1-3.
11 . Wheat grain from the wheat plant of claim 1 .
12 . Flour comprising the wheat grain of claim 11 .
13 . A food product comprising a component of the wheat plant of claim 1 .
14 . A wheat seed, plant part or progeny thereof from the wheat plant of claim 1 .
15 . A wheat plant comprising a mutation in a WPBF gene in B and D genomes, wherein the mutations contribute to grain from said wheat plant having reduced gluten as compared to grain from a wild type plant.
16 . The wheat plant of claim 15 , wherein the mutation in the B genome results in an alteration of a tryptophan to a stop codon at amino acid position of 70 (W70*) of SEQ ID No. 3.
17 . The wheat plant of claim 15 , wherein the mutation in the D genome results in a cysteine to tyrosine mutation at amino acid position 63 (C63Y) of SEQ ID No. 9.
18 . Wheat grain from the wheat plant of claim 15 .
19 . Flour comprising the wheat grain of claim 18 .
20 . A food product comprising a component of the wheat plant of claim 15 .
21 . A wheat seed, plant part or progeny thereof from the wheat plant of claim 15 .