CELL
The present invention relates to a cell comprising a chimeric antigen receptor (CAR) and a constitutively active or inducible Signal Transducer and Activator of Transcription (STAT) molecule.
1 - 33 . (canceled)
34 . A cell which comprises a CAR and a constitutively active or an inducible Janus Kinase (JAK) molecule.
35 . A cell according to claim 34 wherein the JAK molecule is constitutively active.
36 . A cell according to claim 35 wherein the JAK molecule comprises a Gain of Function (GOF) mutation.
37 . A cell according to claim 35 wherein the JAK molecule comprises a first JAK polypeptide linked to a second JAK polypeptide by a linker sequence.
38 . A cell according to claim 35 wherein the JAK molecule comprises a first polypeptide comprising a first dimerizing domain (DD) and a second polypeptide comprising a second DD, which second DD specifically binds to the first DD.
39 . A cell according to claim 34 , wherein the JAK molecule is inducible.
40 . A cell according to claim 39 , wherein the JAK molecule comprises a first polypeptide comprising a first dimerizing domain (DD) and a second polypeptide comprising a second DD, which second DD specifically binds to the first DD; and wherein the presence of an agent causes dimerization of the first and second DD and induces activation of the JAK molecule.
41 . A cell according to claim 39 , wherein the JAK molecule comprises a first polypeptide comprising a first dimerizing domain (DD) and a second polypeptide comprising a second DD, which second DD specifically binds to the first DD; and wherein the presence of an agent causes dissociation of the first and second DD and induces non-activation of the JAK molecule.
42 . A nucleotide construct comprising a first nucleotide sequence encoding a CAR and a second nucleotide sequence encoding a constitutively active or inducible JAK molecule.
43 . A vector comprising a nucleotide construct according to claim 42 .
44 . A method for making a cell according to claim 34 , which comprises the step of introducing into a cell a nucleic acid sequence encoding the constitutively active or inducible JAK molecule.
45 . A pharmaceutical composition comprising a plurality of cells according to claim 34 .
46 . A method for treating and/or preventing a disease, which comprises the step of administering a pharmaceutical composition according to claim 45 to a subject.