TEST CARD FOR ASSAY AND METHOD OF MANUFACTURING SAME
Disclosed herein is a test card including at least one fluid inlet port, at least one microchannel, and a plurality of target zones, where the test card is configured to perform a plurality of assays in the plurality of target zones. Also described herein is a method of using the test card to perform a plurality of assays on a fluid sample in the plurality of target zones.
1 . A method of analyzing a fluid sample, the method comprising:
receiving, at a test card, a fluid sample, the test card including:
at least one fluid inlet port;
a plurality of target zones; and
at least one microchannel fluidically coupling the at least one fluid inlet port and the plurality of target zones; and
performing a plurality of assays on the fluid sample in the plurality of target zones.
2 . The test card according to claim 1 , wherein the plurality of assays comprise a plurality of different assays.
3 . The method according to claim 1 , wherein the plurality of assays include at least one of (a) a nucleic acid amplification assay such as a polymerase chain reaction (“PCR”) assay, (b) an immunoassay such as an enzyme-linked immunosorbent assay (“ELISA”), (c) an ion selective assay such as an agglutination-PCR assay, or (d) an image cytometry assay.
4 . The method according to claim 1 , wherein the plurality of assays include at least one of (a) a capacitance-based assay, (b) a fluorescence-based assay, or (c) a colorimetric-based assay.
5 . The method according to claim 1 , wherein the plurality of target zones include at least two target zones in parallel and/or at least two target zones in series.
6 . The method according to claim 1 , wherein at least two of the plurality of assays are performed in the plurality of target zones at the same time.
7 . The method according to claim 1 , wherein at least two of the plurality of assays are performed in the plurality of target zones at different times.
8 . The method according to claim 1 , wherein the plurality of assays include at least three assays.
9 . The method according to claim 1 , wherein the test card further includes a plurality of fluid detection zones, and wherein the method further comprises detecting a presence of the fluid in at least one of the plurality of fluid detection zones.
10 . The method according to claim 1 , wherein the test card further includes a plurality of sets of electrodes, wherein each set of electrodes is aligned with one of the plurality of target zones, and wherein the method further comprises performing at least one of heating or sensing using at least one of the plurality of sets of electrodes.
11 . The method according to claim 1 , further comprising moving the fluid sample through the at least one microchannel.
12 . The method according to claim 1 , further comprising moving the fluid sample through the at least one microchannel from the at least one fluid inlet port to the plurality of target zones.
13 . The method according to claim 1 , further comprising moving the fluid sample through the at least one microchannel by applying pressure.
14 . The method according to claim 1 , wherein the test card includes multiple fluid inlet ports.
15 . The method according to claim 1 , wherein the test card further includes at least one fluid outlet port.