SYNBIOTIC SYSTEM OF ENCAPSULATED PROBIOTICS AND PREBIOTICS WITH TARGETED DELIVERY FUNCTION FOR COLONIZATION AND NUTRIENTS BIOCONVERSION AND THE METHOD OF MAKING THE SAME
A synbiotic system of encapsulated probiotics and prebiotics for targeted delivery for colonization functions, comprising a probiotics core enclosed with an inner layer of protein-poly/oligosaccharide complex binder as an adhesion promoter that facilitate the attachment onto intestinal epithelial cells, and as a prebiotic promoting the growth of the enclosed probiotics upon release from the outer coating; and an outer layer of natural emulsion system coating on the synbiotic complex to minimize the involvement of water to effectively encapsulate and protect the probiotics. This system is feasible for upscale manufacturing using freeze-drying technology, and can be produced in powder form, suitable for consumption as dietary supplements as well as addition into any food in dry form. The system possesses nutrients bioconversion capability of soy isoflavones to their bioactive aglycone counterparts, as well as releases the bound bioactive phenolic acids from grains that are bioavailable for health promoting effects.
1 . A bilayered synbiotic system for targeted delivery of probiotics, comprising:
an emulsion-based outer layer;
a prebiotic and mucoadhesive binder inner layer; and
a probiotics core;
wherein the emulsion-based outer layer comprises at least one natural emulsion system;
wherein the prebiotic and mucoadhesive binder inner layer comprises an animal-based or plant-based protein hydrolysate and at least one polysaccharides or oligosaccharides;
wherein the probiotics core comprises at least one live probiotics selected from Lactobacillus, Bifidobacterium, Lactococcus, Leuconostoc, Streptococcus, Enterococcus, Staphylococcus, Saccharomyces, Kluyveromyces , or combinations thereof; and
wherein the system comprises live probiotics in an amount of 10-30% in weight.
2 . The bilayered synbiotic system of claim 1 , wherein the animal-based or plant-based protein hydrolysate is selected from rice peptide, soy peptide, pea peptide, chickpea peptide, whey peptide, egg peptide, casein, milk peptide, zein, bovine serum albumin, animal collagen peptide, fish collagen peptide, or combinations thereof.
3 . The bilayered synbiotic system of claim 1 , wherein the at least one polysaccharides or oligosaccharides is selected from galacto-oligosaccharides (GOS), fructo-oligosaccharides (FOS), inulin, maltodextrin, cellulose, chitin, chitosan, pectin, alginates, konjac, arabinoxylans, or combinations thereof.
4 . The bilayered synbiotic system of claim 1 , wherein the at least one natural emulsion systems is selected from cattle milk, buffalo milk, goat milk, sheep milk, camel milk, yak milk, soy milk, almond milk, coconut milk, oat milk, rice milk, barley milk, nut milk, or combinations thereof.
5 . The bilayered synbiotic system of claim 1 , further comprising minerals selected from chalk, talc, kaolin, zinc oxide, titanium oxide, silicon oxide, or combinations thereof.
6 . The bilayered synbiotic system of claim 1 , wherein the cell adhesion of the synbiotic system to intestinal epithelial cells is at least 70%.
7 . The bilayered synbiotic system of claim 1 , wherein the viability of the probiotics decreases no more than 2 Log CFU/g after 180 days storage at a room temperature of 20-30 degrees Celsius.
8 . The bilayered synbiotic system of claim 1 , wherein the viable count of probiotics decreases by no more than 1 Log CFU/g after 2-hour treatment of pH 2 with pepsin under a temperature of 35-37 degrees Celsius.
9 . The bilayered synbiotic system of claim 8 , wherein the viable count of probiotics further decreases by no more than 1 Log CFU/g after 2-hour treatment of pH 6.8 with bile salt under a temperature of 35-37 degrees Celsius after the 2-hour treatment of pH 2 with pepsin under a temperature of 35-37 degrees Celsius.
10 . The bilayered synbiotic system of claim 1 , wherein the particle size of the bilayered synbiotic system is less than 200 μm.
11 . The bilayered synbiotic system of claim 1 , wherein the encapsulation efficiency of the bilayered synbiotic system is at least 90%.
12 . A method of preparing the bilayered synbiotic system of claim 1 , comprising:
mixing an animal-based or plant-based protein hydrolysate and at least one polysaccharides or oligosaccharides in solution and heat to 100° C. for 15 minutes to produce a protein-poly/oligosaccharide complex solution;
stir-mixing the probiotics with the protein-poly/oligosaccharide complex solution to form a first solution;
stir-mixing the first solution with the at least one natural emulsion system to form a second solution; and
freeze-drying the second solution and grinding the crystalline to obtain the bilayered synbiotic system in the form of powder.