Retroviral and Lentiviral Vectors
The present invention provides a retroviral or lentiviral vector having a viral envelope which comprises: (i) a mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain and a transmembrane domain; and/or (ii) a cytokine-based T-cell activating transmembrane protein which comprises a cytokine domain and a transmembrane domain, wherein the mitogenic or cytokine-based T-cell activating transmembrane protein is not part of a viral envelope glycoprotein. When cells such as T-cells of Natural Killer cells are transduced by such a viral vector, they are simultaneously activated by the mitogenic T-cell activating transmembrane protein and/or the cytokine-based T-cell activating transmembrane protein.
1 .- 26 . (canceled)
27 . A kit for making a retroviral or lentiviral vector having a viral envelope that comprises a first mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD3 and comprises a transmembrane domain, and a second mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD28 and comprises a transmembrane domain, the kit comprising:
(a) a host cell which expresses at the cell surface, the first and second mitogenic T-cell activating transmembrane proteins, wherein the mitogenic T-cell activating transmembrane proteins are not part of a viral envelope glycoprotein,
(b) nucleic acids comprising gag, pol, env and optionally rev, and
(c) a retroviral or lentiviral genome.
28 . A kit for making a packaging cell which comprises:
(a) one or more nucleic acid(s) encoding:
(i) a first mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD3 and comprises a transmembrane domain and
(ii) a second mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD28 and comprises a transmembrane domain, wherein the mitogenic T-cell activating transmembrane proteins are not part of a viral envelope glycoprotein, and
(b) nucleic acids comprising retroviral gag, pol, env and optionally rev genes.
29 . A kit for making a producer cell which comprises:
(a) one or more nucleic acid(s) encoding:
(i) a first mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD3 and comprises a transmembrane domain and
(ii) a second mitogenic T-cell activating transmembrane protein which comprises a mitogenic domain which binds CD28 and comprises a transmembrane domain, wherein the mitogenic T-cell activating transmembrane proteins are not part of a viral envelope glycoprotein,
(b) nucleic acids comprising retroviral gag, pol, env and optionally rev genes, and
(c) a retroviral or lentiviral vector genome.