DECOUPLED THERMODYNAMIC SENSING SYSTEM
Ultrasensitive, decoupled thermodynamic sensing platforms for the molecular-level detection of target analytes are disclosed, wherein the sensors have a heating resistor decoupled from a sensing resistor. Embodiments of the decoupled sensor comprise a metallic microheater resistor on one side of substrate, and a sensor resistor coupled to a catalyst on the other side of the substrate. A sensor array may be provided including a plurality of sensors each having a different catalyst that, when exposed to an analyte, each experience an endothermic reaction, an exothermic reaction, or no reaction. A comparison of the reaction results to data comprising previously obtained reaction results may be used to determine the presence and the identity of the analyte. Advantageously, the decoupled sensors utilize less power and provide greater sensitivity than other-known systems, and may be used to detect and identify a single molecule of an analyte.
1 . A detection device comprising:
a first sensor comprising a first microheater layer, a first sensor layer, and a first catalyst layer in thermal communication with the first sensor layer, the first microheater layer separated from the first sensor layer by a first substrate layer;
a second sensor comprising a second microheater layer and a second sensor layer, the second microheater layer separated from the second sensor layer;
a controller in communication with the first sensor and the second sensor, the controller configured to:
cause a first amount of power to be provided to the first and second microheater layers to heat the first sensor layer to a first setpoint temperature and to heat the second sensor layer to a second setpoint temperature;
in response to an endothermic and/or exothermic reaction at the first and/or second sensor, cause a second amount of power to be applied to the first and/or second microheater layers to maintain the first setpoint temperature and the second setpoint temperature;
determine a measurement of the difference between the first amount of power and the second amount of power, and
determine an existence, identity, and/or concentration of an analyte based on the measurement.