CYTOKINE PROFILING ANALYSIS
This invention relates to methods for predicting a prognosis of a patient with cancer in need of an anti-cancer treatment comprising measuring a cytokine score from a sample obtained from the patient. In some embodiments, the subject is administered an anti-cancer treatment, e.g., an anti-PD-1 antibody, following the cytokine score measurement. In some embodiments, the cancer is lung cancer.
1 . A method of treating a patient in need of an anti-cancer treatment comprising administering to the patient an effective amount of an anti-PD-1 antibody or antigen-binding portion thereof (“anti-PD-1 antibody”) or an effective amount of an anti-PD-L1 antibody or antigen-binding portion thereof (“anti-PD-L1 antibody”);
wherein the patient has been identified as having a cytokine score that is higher than an average cytokine score;
wherein the cytokine score is the sum of a point designated to the level of at least two cytokines in a sample obtained from the patient, wherein the point is X if the sample has a high concentration of a cytokine that is positively associated with overall survival of the patient following the administration of the anti-PD-1 antibody or the anti-PD-L1 antibody or a low concentration of a cytokine that is negatively associated with overall survival of the patient following the administration of the anti-PD-1 antibody or the anti-PD-L1 antibody, wherein the point is Z if the sample has a low concentration of a cytokine that is positively associated with the overall survival of the patient following the administration of the anti-PD-1 antibody or the anti-PD-L1 antibody or a high concentration of a cytokine that is negatively associated with the overall survival of the patient following the administration of the anti-PD-1 antibody or the anti-PD-L1 antibody, and, optionally, wherein the point is Y if the sample has a medium concentration of a cytokine that is either negatively or positively associated with overall survival of the patient following the administration of the anti-PD-1 antibody or the anti-PD-L1 antibody;
wherein the at least two cytokines are selected from the group consisting of MIG, IL-1RA, MMP-3, IL-8, FRTN, ICAM, VWF, MICA, IP-10, CRP, IL-18, VDBP, IL-6, MIP1B, MCP2, ENRAGE, IL-2RA, B2M, RANTES, TNFR2, and any combination thereof.
2 . The method of claim 1 , further comprising measuring the cytokine score from the sample obtained from the subject prior to administering the anti-PD-1 antibody or the anti-PD-L1 antibody is higher than an average cytokine score.
3 . The method of claim 2 , wherein the cytokine score is at least about 1% higher than the average cytokine score.
4 . The method of claim 1 , wherein X is any value, Z is a value that is lower than X, and, optionally, Y is a value that is between X and Z.
5 . The method of claim 1 , wherein Z is 0, Y is 1, and X is 2.
6 . The method of claim 1 , wherein the average cytokine score is
any integer between 1 and 100.
7 . The method of claim 1 , wherein the at least two cytokines comprises three cytokines.
8 . The method of claim 1 , wherein the patient has lung cancer.
9 . The method of claim 8 , wherein the lung cancer is non-small cell lung cancer.
10 . The method of claim 1 , wherein the at least two cytokines are selected from the group consisting of MIG, IL-1RA, MMP-3, IL-8, FRTN, ICAM, VWF, MICA, IP-10, CRP, IL-18, VDBP, IL-6, MIP1B, and any combination thereof.
11 . The method of claim 1 , wherein the at least two cytokines are selected from the group consisting of FRTN, MMP-3, IL-8, MCP2, ENRAGE, IL-2RA, IL-18, VWF, B2M, RANTES, MICA, TNFR2, VDBP, and any combination thereof.
12 . The method of claim 1 , wherein the positively associated cytokines are selected from the group consisting of MIG, IL-1RA, MMP-3, and any combination thereof.
13 . The method of claim 1 , wherein the negatively associated cytokines are selected from the group consisting of IL-8, FRTN, ICAM, VWF, MICA, IP-10, CRP, IL-18, VDBP, IL-6, MIP1B, and any combination thereof.
14 . The method of claim 1 , wherein the positively associated cytokines are B2M, VDBP, or both.
15 . The method of claim 1 , wherein the negatively associated cytokines are selected from the group consisting of FRTN, MMP-3, IL-8, MCP2, ENRAGE, IL-2RA, IL-18, VWF, RANTES, MICA, TNFR2, and any combination thereof.
16 - 17 . (canceled)
18 . The method of claim 1 , wherein the anti-PD-1 antibody is nivolumab.
19 . (canceled)
20 . The method of claim 1 , wherein the anti-PD-L1 antibody is selected from BMS-936559, MPDL3280A, MEDI4736, and MSB0010718C.
21 . The method of claim 1 , wherein the anti-PD-1 antibody is pembrolizumab.
22 . The method of claim 1 , wherein the at least two cytokines comprise four cytokines.
23 . The method of claim 1 , wherein the at least two cytokines comprise five cytokines.