Albumin binding proteins and methods of use
Provided herein are albumin binding proteins and methods of making and using thereof.
1 . A humanized albumin binding protein comprising: a VHH domain, the VHH domain comprising three complementarity determining regions (CDRs), CDR1, CDR2, and CDR3 therein, wherein:
(a) the CDR1 has an amino acid sequence represented by SEQ ID NO: 104;
(b) the CDR2 has an amino acid sequence represented by SEQ ID NO: 141, wherein X 63 is S or T; and
(c) the CDR3 has an amino acid sequence represented by SEQ ID NO: 142, wherein X 101 is T or A.
2 . The humanized albumin binding protein of claim 1 , wherein:
(a) the CDR1 has an amino acid sequence represented by SEQ ID NO: 104;
(b) the CDR2 has an amino acid sequence represented by SEQ ID NO: 105; and
(c) the CDR3 has an amino acid sequence represented by SEQ ID NO: 122.
3 . The humanized albumin binding protein of claim 1 , wherein the VHH domain has an amino acid sequence represented by any one of SEQ ID NOs: 130-136.
4 . The humanized albumin binding protein of claim 1 , further comprising component (D2), wherein D2 is linked to the humanized albumin binding protein of claim 1 (D1) according to a formula, from N-to-C terminus, (a) D1-L-D2, or (b) D2-L-D1, wherein L is a peptide linker, and D2 is a second antigen binding protein or a non-antigen binding moiety.
5 . The humanized albumin binding protein of claim 4 , wherein the peptide linker has an amino acid sequence represented by any one of SEQ ID NOs: 58-73.
6 . The humanized albumin binding protein of claim 4 , wherein D2 comprises an antigen binding protein or non-antigen binding moiety selected from a single chain antibody or binding fragment thereof, an scFv, a bispecific scFv, a single heavy chain, a single light chain, a variable heavy-chain domain, a VHH, a variable light-chain domain, a variable NAR domain, a single chain polypeptide. a F (ab′), a F (ab′)2, a bi-specific F(ab′)2), a F(Ab′)3, a tri-specific F(Ab′)3, an Fv, a DARPin, an Fc domain, an entire Fc, a modified Fc domain, an Fc domain with one or more substitutions relative to wild-type, an Fc fragment, a minibody, a diabody, a dAb fragment, an antibody, a monoclonal antibody, a multispecific binding protein, a bispecific antibody, and combinations thereof.