Compositions and Method of Use for Hair Straightening and Shaping
Peptides are provided herein, wherein the peptide bearing one or more amino acid sequence differences relative to SEQ ID NO: 1, with the amino acid sequence differences comprising at least one cysteine replacement or addition.
1 . A peptide comprising substitution of an at least one amino acid of SEQ ID NO:1 with a cysteine, wherein the cysteine substitution consists of SEQ ID NO:8, SEQ ID NOS: 3-7, or SEQ ID NOS: 9-109, or a variant thereof with at least 70% sequence identity thereto.
2 . A peptide of claim 1 , wherein the cysteine substitution located in the region of SEQ ID NO: 1 consisting of SEQ ID NO:8, SEQ ID NOS: 3-7, or SEQ ID NOS: 9-109 is a V17C substitution relative to the amino acid sequence of SEQ ID NO:1.
3 . The peptide of claim 1 comprising a Q13C substitution relative to amino acid sequence of SEQ ID NO:1.
4 . The peptide of claim 2 comprising an H6C substitution relative to amino acid sequence of SEQ ID NO:1.
5 . The peptide of claim 1 comprising at least one additional cysteine substitution at any other amino acid position relative to SEQ ID NO:1.
6 . The peptide of claim 5 , wherein the at least one additional cysteine substitution is ≥1 and ≤11 amino acids relative to SEQ ID NO:1.
7 . The peptide of claim 1 comprising substitution of at least 3 amino acids of SEQ ID NO: 1 with cysteine.
8 . The peptide of claim 5 , wherein the at least one additional cysteine substitution is ≥3 and ≤11 amino acids relative to SEQ ID NO:1.
9 . The peptide of claim 1 , wherein the peptide consists of the amino acid sequence of any one of SEQ ID NO: 8, SEQ ID NOS: 3-7, or SEQ ID NOS: 9-109.
10 . The peptide of claim 1 comprising the amino acid sequence of SEQ ID NO:8, or a variant thereof with at least 70% sequence identity thereto.
11 . The peptide of claim 1 , wherein a plurality of the peptides contacted with hair, optionally damaged hair, increases hair strength greater than the same number of peptides of SEQ ID NO: 1 contacted with hair under the same conditions.
12 . The peptide of claim 1 , wherein the peptide binds keratin.
13 . The peptide of claim 12 , wherein a keratin-bound peptide is more resistant to washing than a keratin bound peptide consisting of the amino acid sequence of SEQ ID NO:1 contacted with hair under the same conditions.
14 . A composition comprising a plurality of the peptide of claim 1 .
15 . The composition of claim 14 , wherein the composition is a liquid.
16 . The composition of claim 14 comprising about 0.01% to about 0.1% w/w of the peptide.
17 . The composition of claim 14 , wherein the composition is selected from the group consisting of a shampoo, a conditioner, an oil, or a mask.
18 . A method of treating hair, the method comprising: applying the composition of claim 14 to hair.
19 . The method of claim 18 , wherein the applying step occurs subsequent to application of a hair waving formulation, a hair straightening formulation, a hair coloring formulation, or a hair bleaching formulation to the hair.
20 . The method of claim 18 , wherein breakage of the hair is decreased by at least about 5%, 10%, 20%, 30%, 40%, or 50% as compared to when the hair is not treated with the composition following the application of a hair waving formulation, a hair straightening formulation, a hair coloring formulation, or a hair bleaching formulation to the hair.
21 . The peptide of claim 1 , wherein one cysteine substitution is located in the region of SEQ ID NO:1 consisting of SEQ ID NO:2.
22 . The peptide of claim 21 , wherein the one cysteine substitution located in the region of SEQ ID NO:1 consisting of SEQ ID NO:2 is a V17C substitution relative to the amino acid sequence of SEQ ID NO: 1.