HIGH SPEED SCANNING SYSTEMS FOR SUPER RESOLUTION IMAGING
Disclosed herein is a high throughput optical scanning system to generate super resolution images and methods of use. The optical scanning device and methods of use provided herein can allow high throughput scanning of a continuously moving object with a high resolution despite fluctuations in stage velocity. This can aid in high throughput scanning of a substrate, such as a biological chip comprising fluorophores. Also provided herein are improved optical relay systems and scanning optics.
1 .- 37 . (canceled)
38 . An imaging system comprising:
a light source;
one or more imaging sensors operably coupled to an objective lens to capture at least a field of a plurality of fields of a substrate;
a stage to support said substrate, wherein said stage moves along a path;
an encoder operably coupled to said stage;
a position processing module to receive positional information from said encoder and generate a plurality of calculated positions for said stage; and
a scanning mirror coupled to an axis adjacent to said path to scan said field of said plurality of fields of said substrate,
wherein said scan comprises a forward scan and a back scan, and wherein said forward scan and said back scan are generated by said position processing module based on said plurality of calculated positions.
39 . A method of imaging a moving substrate:
providing a substrate on a stage, wherein said substrate comprises a plurality of fields, and wherein an encoder is operably coupled to said stage;
translating said stage along a path;
determining a plurality of positions of said stage from a position processing module electrically coupled to said encoder, wherein said position processing module performs a plurality of calculations based on said plurality of positions of said stage; and
actuating a scanning mirror coupled to an axis adjacent to said path to scan said light source with said scanning mirror along a field of said plurality of fields of said substrate, wherein said scan comprises a forward scan and a back scan generated by said position processing module based on said plurality of calculations.