Aptamers Against Clostridium Difficile
Compositions comprising aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and/or isolating Clostridium difficile spores is provided.
1 .- 20 . (canceled)
21 . An aptamer, the aptamer comprising from position 22 to position 61 of the nucleotide sequence of SEQ ID NO: 6, wherein the aptamer has a binding affinity for CdeC surface protein of Clostridium difficile spore.
22 . An aptamer, the aptamer comprising from position 22 to position 60 of the nucleotide sequence of SEQ ID NO: 10, wherein the aptamer has a binding affinity for CotA surface protein of Clostridium difficile spore.
23 . An aptamer, the aptamer comprising from position 22 to position 61 of the nucleotide sequence of SEQ ID NO: 5, wherein the aptamer has a binding affinity for CotEC Chitinase surface protein of Clostridium difficile spore.
24 . An aptamer, the aptamer comprising from position 22 to position 54 of the nucleotide sequence of SEQ ID NO: 13, wherein the aptamer has a binding affinity for CotE surface protein of Clostridium difficile spore.
25 . The aptamer of claim 21 , wherein the aptamer comprises a detectable label.
26 . The aptamer of claim 25 , wherein the detectable label comprises a fluorophore, a nanoparticle, a radioactive isotope, a biotin, a protein or a combination thereof.
27 . An apparatus for detecting the presence, absence or level of Clostridium difficile spores in a sample, the apparatus comprising:
a support; and
at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 21 .
28 . The apparatus of claim 27 , wherein at least one aptamer comprises a detectable label.
29 . The apparatus of claim 28 , wherein the detectable label comprises a fluorophore, a nanoparticle, a radioactive isotope, a biotin, a protein or a combination thereof.
30 . A composition comprising at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 21 .
31 . The composition of claim 30 , wherein at least one aptamer comprises a detectable label.
32 . The composition of claim 31 , wherein the detectable label comprises a fluorophore, a nanoparticle, a radioactive isotope, a biotin, a protein or a combination thereof.
33 . An apparatus for detecting the presence, absence or level of Clostridium difficile spores in a sample, the apparatus comprising:
a support; and
at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 22 .
34 . A composition comprising at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 22 .
35 . The composition of claim 34 , wherein at least one aptamer comprises a detectable label.
36 . An apparatus for detecting the presence, absence or level of Clostridium difficile spores in a sample, the apparatus comprising:
a support; and
at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 23 .
37 . A composition comprising at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 23 .
38 . The composition of claim 37 , wherein at least one aptamer comprises a detectable label.
39 . An apparatus for detecting the presence, absence or level of Clostridium difficile spores in a sample, the apparatus comprising:
a support; and
at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 24 .
40 . A composition comprising at least one aptamer, wherein the at least one aptamer comprises the aptamer of claim 24 .