Cordyceps Militaris Designated as Strain M2-116-04 Compositions, Methods of Making, Methods of Using, and Methods for Treating a Mammal
Embodiments include a not naturally occurring strain of Cordyceps militaris fungus designated as strain M2-116-04 as deposited with the ATCC Patent Depository under the Budapest Treaty (10801 University Blvd, Manassas, VA 20110), on Jul. 11, 2024, with the unofficial ATCC Patent Deposit Designation No. PTA-127802, and the official deposit date of Jul. 11, 2024, and the official Patent Deposit Designation No. PTA-127802. The isolated mycelium is characterized by a phenotype having an abundance of sporocarps on top of a substrate and/or along a parameter of a bioreactor bag, and also characterized by a phenotype having fruiting initiation at or near 18 days after inoculation, a phenotype having sporocarps maturing at or near 55 days after inoculation, and/or a phenotype having a 10-to-15-day colonization time on hulled oats. Applications include functional foods, prebiotic formulations, and immune support products, with the strain available in powder, capsule, or drink mix forms.
1 . A not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 as deposited with the ATCC Patent Depository under the Budapest Treaty (10801 University Blvd, Manassas, VA 20110), on Jul. 11, 2024, with the unofficial ATCC Patent Deposit Designation No. PTA-127802, and the official deposit date of Jul. 11, 2024, and the official Patent Deposit Designation No. PTA-127802.
2 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having an abundance of sporocarps on top of a substrate and along a parameter of a bioreactor bag.
3 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having fruiting initiation at or near 18 days after inoculation.
4 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having sporocarps maturing at or near 55 days after inoculation.
5 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a 10-to-15-day colonization time on hulled oats.
6 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype optimized to grow on oats.
7 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a beta-glucan content of approximately 20% to 25% as represented as a percentage of a total mycelium and sporocarps.
8 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a beta-glucan content of approximately 26% to 31% as represented as a percentage of a total mycelium and sporocarps.
9 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a beta-glucan content of approximately 32% to 37% as represented as a percentage of a total mycelium and sporocarps.
10 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a beta-glucan content of approximately 38% to 43% as represented as a percentage of a total mycelium and sporocarps.
11 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a beta-glucan content of approximately 44% to 49% as represented as a percentage of a total mycelium and sporocarps.
12 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , characterized by a phenotype having a cordycepin content of approximately 0.4 milligrams per gram to 1.1 milligrams per gram as represented relative to a total mycelium and sporocarps.
13 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , in a powder form.
14 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , in a capsule form.
15 . The not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04 of claim 1 , in a drink mix form for cold and hot beverages applications.
16 . A method for treating a mammal to improve immune functioning of the mammal, the method comprising administering an effective dose of Cordyceps militaris mushroom powder comprising Cordyceps militaris mycelium and sporocarps.
17 . The method of claim 16 , wherein the effective dose is administered once every 12 days over 8 separate consecutive 12-day intervals.
18 . The method of claim 16 , wherein the Cordyceps militaris mushroom powder comprises not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04.
19 . The method of claim 16 , further comprising the effective dose being 500 mg of the Cordyceps militaris mushroom powder per kg of body weight of the mammal.
20 . The method of claim 16 , further comprising the effective dose being 1000 mg of the Cordyceps militaris mushroom powder per kg of body weight of the mammal.
21 . The method of claim 16 , further comprising the effective dose being 2000 mg of the Cordyceps militaris mushroom powder per kg of body weight of the mammal.
22 . The method of claim 16 , further comprising the mammal being a human.
23 . A method for treating a mammal to improve gut functioning of the mammal, the method comprising administering an effective dose of Cordyceps militaris mushroom powder comprising not naturally occurring Cordyceps militaris fungus designated as strain M2-116-04, the mushroom powder comprising Cordyceps militaris mycelium and sporocarps and being a prebiotic.