WORKING ELECTRODE OF A CONTINUOUS BIOLOGICAL SENSOR

1 . A method for making an enzyme membrane for a working electrode of a continuous biological monitor, the method comprising:

making an aqueous silicone dispersion;

making an acrylic polyol emulsion;

mixing the silicone dispersion and the acrylic polyol emulsion to make a base emulsion;

adding an enzyme to the base emulsion to create an enzyme/base emulsion dispersion, the enzyme being selected according to a biological function to be monitored;

applying the enzyme/base emulsion dispersion to the working electrode; and

curing the applied enzyme/base emulsion dispersion.

2 . The method according to claim 1 , wherein the enzyme is glucose oxidase (GOx) and the biological function is glucose level.

3 . The method according to claim 1 , further comprising adding a hydrophile to the enzyme/base emulsion dispersion prior to the applying.

4 . The method according to claim 3 , wherein the hydrophile is polyvinylpyrrolidone (PVP), polyethylene oxide (PEO) or silane-PEO (Si-PEO).

5 . The method according to claim 1 , further comprising adding a cross-linker to the enzyme/base emulsion dispersion prior to the applying.

6 . The method according to claim 5 , wherein the cross-linker is glutaraldehyde or polyaziridine.

7 . The method according to claim 1 , further comprising adding imidoesters, hydroxysuccinimide, carbodilite, melamines, epoxies, benzoyl peroxide or dicumyl peroxide to the enzyme/base emulsion dispersion prior to the applying.

8 . The method according to claim 1 , wherein the applying comprises dipping, spraying, depositing or printing.

9 . The method according to claim 1 , wherein the curing comprises moving air, applying heat, or applying a vacuum.

10 . The method according to claim 1 , wherein a ratio of the silicone dispersion to the acrylic polyol emulsion is about 1 to 1 by volume.

11 . The method according to claim 1 , wherein the enzyme is lactate dehydrogenase, lactate oxidase or hydroxybutyrate dehydrogenase.