ENGINEERED VIRUS
The present invention relates to oncolytic virus comprising: (i) a GM-CSF-encoding gene; and (ii) an immune co-stimulatory pathway activating molecule or an immune co-stimulatory pathway activating molecule-encoding gene.
1 . An oncolytic virus comprising: (i) a GM-CSF-encoding gene; and (ii) an immune co-stimulatory pathway activating molecule or an immune co-stimulatory pathway activating molecule-encoding gene.
2 . The virus of claim 1 , wherein the immune co-stimulatory pathway activating molecule-encoding gene encodes CD40 ligand (CD40L), ICOS ligand, GITR ligand, 4-1-BB ligand, OX40 ligand, TL1A, CD30 ligand, CD27 or flt3 ligand or a modified version of any of these.
3 . The virus of claim 1 or 2 , wherein the immune co-stimulatory pathway activating molecule-encoding gene encodes CD40 ligand, GITR ligand, 4-1-BB ligand, OX40 ligand, ICOS ligand or a modified version of any of these.
4 . The virus of claim 1 , wherein the immune co-stimulatory pathway activating molecule-encoding gene encodes a CTLA-4 inhibitor.
5 . The virus of claim 4 , wherein the CTLA-4 inhibitor is a CTLA-4 antibody or fragment thereof.
6 . The virus of any one of claims 1 to 5 , further comprising a fusogenic protein-encoding gene.
7 . The virus of claim 6 where the fusogenic protein is selected from the group consisting of vesicular stomatitis virus (VSV) G-protein, syncitin-1, syncitin-2, simian virus 5 (SV5) F-protein, measles virus (MV) H-protein, MV F-protein, respiratory syncytial virus (RSV) F-protein and a glycoprotein from gibbon ape leukemia virus (GALV), murine leukemia virus (MLV), Mason-Pfizer monkey virus (MPMV) or equine infectious anaemia virus (EIAV) from which the R peptide has been deleted.
8 . The virus of claim 6 or 7 , wherein the fusogenic protein is the glycoprotein from gibbon ape leukemia virus (GALV) and has the R transmembrane peptide mutated or removed (GALV-R-).
9 . The virus of any one of the preceding claims , which encodes more than one immune co-stimulatory pathway activating molecule.
10 . The virus of any one of the preceding claims , which is derived from a clinical isolate of a virus.
11 . The virus of any one of the preceding claims , which is a modified clinical isolate of a virus, wherein the clinical isolate kills two or more tumor cell lines more rapidly and/or at a lower dose in vitro than one or more reference clinical isolates of the same species of virus.
12 . The virus of claim 10 or 11 , wherein the clinical isolate is
strain RH018A having the provisional accession number ECCAC 16121904;
strain RH004A having the provisional accession number ECCAC 16121902;
strain RH031A having the provisional accession number ECCAC 16121907;
strain RH040B having the provisional accession number ECCAC 16121908;
strain RH015A having the provisional accession number ECCAC 16121903;
strain RH021A having the provisional accession number ECCAC 16121905;
strain RH023A having the provisional accession number ECCAC 16121906; or
strain RH047A having the provisional accession number ECCAC 16121909.
13 . The virus of any one of claims 1 to 11 , which is selected from the group consisting of herpes viruses, pox viruses, adenoviruses, retroviruses, rhabdoviruses, paramyxoviruses and reoviruses.
14 . The virus of any one of the preceding claims , which is a herpes simplex virus (HSV).
15 . The virus of claim 14 which is a HSV1.
16 . The virus of claim 15 , wherein the HSV:
(a) does not express functional ICP34.5;
(b) does not express functional ICP47; and/or
(c) expresses the US11 gene as an immediate early gene.
17 . The virus of any one of claims 14 to 16 , wherein the GM-CSF-encoding gene and an immune co-stimulatory pathway activating molecule-encoding gene are inserted into the ICP34.5 encoding locus, either by insertion, or partial or complete deletion, in a back to back orientation in relation to each other, each under separate regulatory control.
18 . The virus of any one of the preceding claims , wherein the sequence of a gene encoding GM-CSF and/or the sequence of the gene encoding an co-immune stimulatory pathway activating molecule is codon optimized so as to increase expression levels in target cells.
19 . A virus which expresses three heterologous genes, wherein each of the three heterologous genes is driven by a different promoter selected from the CMV promoter, the RSV promoter, the SV40 promoter (SEQ ID) and a retroviral LTR promoter.
20 . A virus according to any one of the preceding claims , which expresses three heterologous genes, wherein each of the three heterologous genes is driven by a different promoter selected from the CMV promoter, the RSV promoter, the SV40 promoter and a retroviral LTR promoter.
21 . The virus of claim 19 or 20 , which expresses four heterologous genes driven by each of the CMV promoter, the RSV promoter, the SV40 promoter and a retroviral LTR promoter, respectively.
22 . The virus of any one of claims 19 to 21 , where the retroviral LTR is from MMLV.
23 . A virus which expresses three heterologous genes, wherein each of the three heterologous genes is terminated by a different poly adenylation sequence selected from the BGH, SV40, HGH and RBG poly adenylation sequences.
24 . A virus according to any one of the preceding claims , which expresses three heterologous genes, wherein each of the three heterologous genes is terminated by a different poly adenylation sequence selected from the BGH, SV40, HGH and RBG poly adenylation sequences.
25 . The virus of claim 23 or 24 , which expresses four heterologous genes terminated by each of the BGH, SV40, HGH and RBG poly adenylation sequences, respectively.
26 . The virus of any one of claims 19 to 25 which is
(a) a HSV;
(b) a HSV1; or
(c) a pox virus.
27 . A pharmaceutical composition comprising a virus according to any one of claims 1 to 26 and a pharmaceutically acceptable carrier or diluent.
28 . The virus of any one of claims 1 to 26 for use in a method of treating the human or animal body by therapy.
29 . The virus of any one of claims 1 to 26 for use in a method of treating cancer.
30 . The virus for use according to claim 29 , wherein the method comprises administering a further anti-cancer agent.
31 . The virus for use according to claim 30 , wherein the further anti-cancer agent is selected from an agent targeting an immune co-inhibitory or immune co-stimulatory pathway, radiation and/or chemotherapy, an agent that targets a specific genetic mutation which occurs in tumors, an agent intended to induce an immune response to one or more tumor antigen(s) or neoantigen(s), a cellular product derived from T cells or NK cells, an agent intended to stimulate the STING, cGAS, TLR or other innate immune response and/or inflammatory pathway, a second virus optionally an oncolytic virus, and combinations thereof.
32 . The virus for use according to claim 30 or 31 , wherein the agent targeting an immune co-inhibitory pathway is a CTLA-4 inhibitor, a PD-1 inhibitor, a PD-L1 inhibitor, a LAG-3 inhibitor, a TIM-3 inhibitor, a VISTA inhibitor, aCSF1R inhibitor, an IDO inhibitor, a KIR inhibitor, a SLAMF7 inhibitor, a CEACAM1 inhibitor or a CD47 inhibitor, and/or the agent targeting an immune co-stimulatory pathway is a GITR agonist, a 4-1-BB agonist, an OX40 agonist, a CD40 agonist or an ICOS agonist.
33 . The virus for use according to any one of claims 30 to 32 , wherein the further anti-cancer agent is an antibody.
34 . The virus for use according to any one of claims 30 to 33 , wherein the method comprises administering an inhibitor of the indoleamine 2,3-dioxygenase (IDO) pathway and a further antagonist of an immune co-inhibitory pathway, or an agonist of an immune co-stimulatory pathway.
35 . The virus for use according to any one of claims 29 to 34 , wherein the virus and the further anti-cancer agent(s) are administered separately.
36 . The virus for use according to any one of claims 29 to 34 , wherein the virus and the further anti-cancer agent(s) are administered concurrently.
37 . The virus for use according to any one of claims 29 to 36 , wherein the cancer is a solid tumor.
38 . A product of manufacture comprising a virus according to any one of claims 1 to 26 in a sterile vial, ampoule or syringe.
39 . A method of treating cancer, which comprises administering a therapeutically effective amount of the virus of any one of claims 1 to 26 or a pharmaceutical composition according to claim 27 to a patient in need thereof.
40 . A method according to claim 39 , which further comprises administering a therapeutically effective amount of a further anti-cancer agent to a patient in need thereof.
41 . A method according to claim 40 , wherein the further anti-cancer agent is selected from the group consisting of an agent targeting an immune co-inhibitory or immune co-stimulatory pathway, radiation and/or chemotherapy, an agent that targets a specific genetic mutation which occurs in tumors, an agent intended to induce an immune response to one or more tumor antigen(s) or neoantigen(s), a cellular product derived from T cells or NK cells, an agent intended to stimulate the STING, cGAS, TLR or other innate immune response and/or inflammatory pathway, a second virus optionally an oncolytic virus, and combinations thereof.
42 . A method according to claim 41 , wherein the agent targeting an immune co-inhibitory pathway is a CTLA-4 inhibitor, a PD-1 inhibitor, a PD-L1 inhibitor, a LAG-3 inhibitor, a TIM-3 inhibitor, a VISTA inhibitor, aCSF1R inhibitor, an IDO inhibitor, a KIR inhibitor, a SLAMF7 inhibitor, a CEACAM1 inhibitor or a CD47 inhibitor, and/or the agent targeting an immune co-stimulatory pathway is a GITR agonist, a 4-1-BB agonist, an OX40 agonist, a CD40 agonist or an ICOS agonist.
43 . A method according to claim 41 or 42 , wherein the further anti-cancer agent comprises an antibody.
44 . A method according to any one of claims 40 to 43 , wherein the virus and the further anti-cancer agent(s) are administered separately.
45 . A method according to any one of claims 40 to 43 , wherein the virus and the further anti-cancer agent(s) are administered concurrently.
46 . A method according to any one of claims 40 to 45 , wherein the cancer is a solid tumor.
47 . Use of the virus of any one of claims 1 to 26 in the manufacture of a medicament for use in a method of treating cancer.
48 . Use according to claim 47 , wherein the method comprises administering a further anti-cancer agent.