MILK PRODUCTION AND COMPOSITIONAL CHARACTERISTICS WITH NOVEL RUMINOCOCCUS BOVIS
The disclosure relates to isolated microorganisms—including novel strains of the microorganisms such as Ruminococcus bovis sp. nov.—microbial ensembles, and compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial ensembles, and compositions comprising the same, in methods for modulating the production and yield of milk and milk components in ruminants. In particular aspects, the disclosure provides methods of increasing desirable components of milk in ruminants. Furthermore, the disclosure provides for methods of modulating the rumen microbiome.
1 . An orally deliverable composition, comprising:
a) Ruminococcus bovis comprising a 16S nucleic acid sequence of SEQ ID NO: 2108; and
b) a carrier suitable for oral ruminant administration.
2 . The composition of claim 1 , wherein the Ruminococcus bovis is deposited as PTA-125917, NRRL B-67764, TSD-225, or NCTC 14479.
3 . The composition of claim 1 , wherein the composition further comprises:
a) one or more bacteria comprising a 16S nucleic acid sequence sharing at least about 97% sequence identity to any one of SEQ ID NOs: 1-30 and 2045-2103; and/or
b) one or more fungi comprising an ITS nucleic acid sequence sharing at least about 97% sequence identity to any one of SEQ ID NOs: 31-60 and 2104-2107.
4 . The composition of claim 1 , wherein the composition further comprises:
a) a Clostridium sp. comprising a 16S nucleic acid sequence sharing at least about 97% sequence identity to SEQ ID NO: 28;
b) a Pichia sp. comprising an ITS nucleic acid sequence sharing at least about 97% sequence identity to SEQ ID NO: 32; and/or
c) a Butyrivibrio sp. comprising a 16S nucleic acid sequence sharing at least about 97% sequence identity to SEQ ID NO: 2067.
5 . The composition of claim 1 , wherein the composition further comprises:
a) a Clostridium sp. with a deposit accession number of NRRL B-67248;
b) a Pichia sp. with a deposit accession number of NRRL Y-67249; and/or
c) a Butyrivibrio sp. with a deposit accession number of NRRL B-67347.
6 . The composition of claim 1 , wherein the Ruminococcus bovis comprises one or more mutations in the whole genome.
7 . The composition of claim 6 , wherein the one or more mutations are selected from the group consisting of:
a) a G→T substitution at position 297 of SEQ ID NO: 2109;
b) a CC→TA substitution at positions 301-302 of SEQ ID NO: 2111;
c) a T→G substitution at position 307 of SEQ ID NO: 2111;
d) a −A deletion at position 300 of SEQ ID NO: 2113;
e) a CCA→TTC substitution at positions 116-118 of SEQ ID NO: 2115;
f) a +T insertion between positions 105-106 of SEQ ID NO: 2117;
g) a C→T substitution at position 298 of SEQ ID NO: 2119;
h) a C→A substitution at position 298 of SEQ ID NO: 2121; and
i) a +AC insertion between positions 43-44 of SEQ ID NO: 2123.
8 .- 19 . (canceled)
20 . The composition of claim 1 , wherein the composition is formulated to protect Ruminococcus bovis from external stressors prior to entering the gastrointestinal tract of the ruminant.
21 . The composition of claim 1 , wherein the ruminant is a cow.
22 . The composition of claim 1 , wherein a ruminant administered the composition exhibit an increase in milk production that leads to an increase in milk yield or an increase in energy-corrected milk.
23 . The composition of claim 1 , wherein a ruminant administered the composition exhibits an improved milk compositional characteristic selected from the group consisting of: an increase in milk fat(s), an increase in milk protein(s), an increase of carbohydrates in milk, an increase of vitamins in milk, an increase of minerals in milk, or combinations thereof.
24 . The composition of claim 1 , wherein a ruminant administered the composition exhibits at least one improved phenotypic trait, selected from the group consisting of: an improved efficiency in feed utilization, improved digestibility, an increase in polysaccharide and lignin degradation, an increase in fatty acid concentration in the rumen, pH balance in the rumen, a reduction in methane emissions, a reduction in manure production, improved dry matter intake, an improved efficiency of nitrogen utilization, or combinations thereof.
25 . The composition of claim 1 , wherein the composition is formulated to protect the Ruminococcus bovis from oxidative stress or moisture.
26 .- 27 . (canceled)
28 . The composition of claim 1 , wherein the composition is combined with food.
29 . (canceled)
30 . The composition of claim 1 , wherein the composition is combined with cereal, starch, oilseed cake, vegetable waste, hay, haylage, silage, livestock feed, forage, fodder, beans, grains, micro-ingredients, fermentation compositions, mixed ration, total mixed ration, or a mixture thereof.
31 . The composition of claim 1 , wherein the composition is formulated as a solid, liquid, or mixture thereof.
32 . The composition of claim 1 , wherein the composition is formulated as a pellet, capsule, granulate, or powder.
33 . The composition of claim 1 , wherein the composition is combined with water, medicine, vaccine, or a mixture thereof.
34 . The composition of claim 1 , wherein the composition is encapsulated.
35 . The composition of claim 34 , wherein the composition is encapsulated in a polymer or carbohydrate.
36 . The composition of claim 1 , wherein the Ruminococcus bovis is present in the composition in an amount of at least 10 2 cells.
37 . A method for increasing milk production or improving milk compositional characteristics in a ruminant, the method comprising orally administering to a ruminant an effective amount of the composition of claim 1 .
38 .- 40 . (canceled)
41 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production that leads to a measured increase in milk yield.
42 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an increase in milk production and improved milk compositional characteristics that leads to a measured increase in energy-corrected milk.
43 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits an improved milk compositional characteristic selected from the group consisting of: an increase in milk fat(s), an increase in milk protein(s), an increase of carbohydrates in milk, an increase of vitamins in milk, an increase of minerals in milk, or combinations thereof.
44 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement exhibits at least a 1% increase, at least a 10% increase, or at least a 20% increase in the average production of: milk fat(s), milk protein(s), energy-corrected milk, or combinations thereof.
45 .- 46 . (canceled)
47 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits at least one improved phenotypic trait, selected from the group consisting of: an improved efficiency in feed utilization, improved digestibility, an increase in polysaccharide and lignin degradation, an increase in fatty acid concentration in the rumen, pH balance in the rumen, a reduction in methane emissions, a reduction in manure production, improved dry matter intake, an improved efficiency of nitrogen utilization, or combinations thereof.
48 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits a shift in the microbiome of the rumen.
49 . The method according to claim 37 , wherein the ruminant administered the effective amount of the ruminant supplement, further exhibits a shift in the microbiome of the rumen, wherein a population of microbes present in the rumen before administration of the ruminant supplement increase in abundance after administration of the ruminant supplement, and/or wherein a population of microbes present in the rumen before administration of the ruminant supplement decrease in abundance after administration of the ruminant supplement.
50 .- 56 . (canceled)