METHODS OF USING ZSCAN4 FOR REJUVENATING HUMAN CELLS
The present disclosure relates to methods for increasing telomere length in one or more human cells and/or increasing genome stability of one or more human cells, for example by contacting one or more human cells with an agent that increases expression of Zscan4 in the one or more human cells. Methods of treating a subject in need of telomere lengthening, treating a disease or condition associated with a genomic and/or chromosome abnormality, of rejuvenating one or more human cells, of rejuvenating tissues or organs, and of rejuvenating a subject in need thereof, for example by contacting one or more human cells in the subject with an agent that increases expression of Zscan4, or by administering to a subject in need thereof, an agent that increases expression of Zscan4 are also provided.
1 - 32 . (canceled)
33 . A method of treating idiopathic pulmonary fibrosis, comprising administering to a lung of a subject in need thereof an agent that increases expression of Zscan4.
34 - 104 . (canceled)
105 . The method of claim 33 , wherein the administering comprises having the subject inhale the agent.
106 . The method of claim 33 , wherein the agent comprises an isolated nucleic acid molecule encoding human Zscan4.
107 . The method of claim 106 , wherein the nucleic acid molecule comprises a vector.
108 . The method of claim 107 , wherein vector is a Sendai virus vector.
109 . The method of claim 108 , wherein the Sendai virus vector is a temperature-sensitive Sendai virus vector.
110 . The method of claim 33 , wherein the nucleic acid molecule is a synthetic mRNA.
111 . The method of claim 110 , wherein the synthetic mRNA is produced from a DNA template encoding the human Zscan4 protein by in vitro transcription in the presence of at least one modified nucleotide triphosphate.