IP Library › Patent Application 19457836
Patent Application
App. No. 19/457,836

COMPOSITIONS, METHODS, KITS, CARTRIDGES, AND SYSTEMS FOR SEQUENCING REAGENTS

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Quick Facts
Patent No.
US None
App. No.
19/457,836
Abstract

The present disclosure relates to a composition including one or more modified nucleotide, wherein the modified nucleotide comprises a purine or pyrimidine base and a sugar moiety having a 3′-hydroxy blocking group, and a radical scavenger, wherein the composition is lyophilised. The present disclosure further relates to a composition including one or more functional protein; one or more functional protein activator; and one or more non-reducing sugar, wherein the composition is lyophilised. Also disclosed are methods of rehydration of one or more compositions described herein and kits including one or more compositions described herein. Further disclosed are cartridges including a flow cell comprising one or more reagent reservoirs, where the one or more reagent reservoirs include one or more compositions described herein.

Claims (24)

1 . A composition comprising:

one or more functional protein;

one or more functional protein activator; and

one or more non-reducing sugar, wherein the composition is lyophilised.

2 . The composition of claim 1 , wherein the functional protein comprises a polymerase, a recombinase, a DNA binding protein, or a combination thereof.

3 . The composition of claim 1 , wherein the functional protein activator is magnesium, a magnesium salt, or other magnesium derivative, or a combination thereof.

4 . The composition of claim 1 , wherein the one or more non-reducing sugar is trehalose, sucrose, inositol, maltodextrin, or dextran, mannitol, raffinose, cyclodextrin, melezitose, sorbitol, or a combination thereof.

5 . The composition of claim 1 , wherein the composition comprises at least about 5 wt. % non-reducing sugar.

6 . The composition of claim 1 , wherein the composition comprises about 5 wt % or less non-reducing sugar.

7 . The composition of claim 1 , wherein the composition is a microsphere, a cake, or a combination thereof.

8 . The composition of claim 7 , wherein the composition has a cross-section of between about 0.1 mm and about 25 mm.

9 . The composition of claim 7 , wherein, when the composition is a microsphere, and the microsphere is spherical, elliptical, or toroidal.

10 . The composition of claim 1 , wherein the composition comprises less than about 1 wt. % glycerol.

11 . The composition of claim 1 , wherein the composition comprises about 5 wt. % or less of a crowding agent.

12 . The composition of claim 11 , wherein the crowding agent comprises polyethylene glycol (PEG), polyvinyl pyrrolidone, dextran, maltodextrin, ficoll, polyacrylamide, or a combination thereof.

13 . The composition of claim 1 , further comprising:

one or more recombinase loading protein, nucleotide triphosphate (dNPT), ATP, ATP analog, buffer, reducing agent, creatine kinase, or a combination thereof.

14 . The composition of claim 2 , wherein the polymerase is a DNA polymerase.

15 . The composition of claim 2 , wherein the DNA binding protein is a single-stranded DNA binding protein.

16 . The composition of claim 1 , wherein the moisture content is below about 10 wt. %.

17 . The composition of claim 1 , wherein the one or more non-reducing sugar comprises two or more non-reducing sugars.

18 . The composition of claim 17 , wherein the two or more non-reducing sugars comprise trehalose and mannitol.

19 . The composition of claim 17 , wherein the two or more non-reducing sugars comprise trehalose and inositol.

20 . The composition of claim 17 , wherein the two or more non-reducing sugars comprise trehalose and sorbitol.