IP Library › Patent Application 19534304
Patent Application
App. No. 19/534,304

NUCLEIC ACID LOADED RED BLOOD CELL EXTRACELLULAR VESICLES

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Quick Facts
Patent No.
US None
App. No.
19/534,304
Abstract

An extracellular vesicle loaded with a nucleic acid cargo and method for preparing the loaded vesicle is disclosed.

Claims (23)

1 . A red blood cell extracellular vesicle (RBCEV) loaded with a DNA cargo.

2 . The RBCEV of claim 1 , wherein the DNA cargo is single stranded and has a length of at least 250 bases.

3 . The RBCEV of claim 1 , wherein the DNA cargo is single stranded and has a length of at least 2000 bases or 2000-30000 bases or more.

4 . The RBCEV of claim 1 , wherein the DNA cargo is double stranded and has a length of at least 250 base pairs.

5 . The RBCEV of claim 1 , wherein the DNA cargo is double stranded and has a length of at least 2000 base pairs or 2000-17000 base pairs or more.

6 . The RBCEV of claim 1 , wherein the DNA cargo is an expression vector comprising a gene encoding a protein or peptide.

7 . The RBCEV of claim 1 , wherein the DNA cargo is circular.

8 . The RBCEV of claim 1 , wherein the DNA cargo is a minicircle or plasmid.

9 . The RBCEV of claim 1 , wherein the DNA cargo is linear.

10 . The RBCEV of claim 1 , wherein the DNA cargo is in the lumen of the RBCEV.

11 . The RBCEV of claim 1 , wherein the RBCEV is derived or obtained from human or mammalian red blood cells.

12 . The RBCEV of claim 1 , wherein the RBCEV is isolated.

13 . An isolated red blood cell extracellular vesicle (RBCEV) containing in the lumen of the RBCEV at least one DNA cargo.

14 . A composition comprising a plurality of RBCEVs according to claim 1 .

15 . The composition of claim 14 , wherein on average each RBCEV in the composition is loaded with at least 1.0 DNA cargo.

16 . The composition of claim 14 , wherein on average each RBCEV in the composition is loaded with at least 2.0 or 3.0 DNA cargoes.

17 . The composition of claim 14 , wherein on average each RBCEV in the composition is loaded with 1.0 to 4.0 DNA cargoes.

18 . The composition of claim 14 , wherein the composition is a pharmaceutical composition or medicament.

19 . The composition of claim 18 , wherein the composition or medicament further comprises a pharmaceutically acceptable carrier, diluent, excipient or stabiliser.

20 . A method of treating a subject in need of treatment, the method comprising administering to the subject a therapeutically effective amount of an RBCEV according to claim 1 .

21 . The method of claim 20 , wherein the method involves treatment of a disease in the subject by expression of a protein or peptide from a gene sequence of the DNA cargo.

22 . The method of claim 21 , wherein the treatment comprises prevention and/or amelioration of the disease.

23 .- 42 . (canceled)