Methanogen Detection Device and System and Method of Detecting Methanogen
A methanogen detection device including a sample inlet, a sample transport channel in fluid communication with the sample inlet, and a capillary flow channel in fluid communication with the sample transport channel. The capillary flow channel is configured to support flow of a sample including methanogens and each of the methanogens includes Cofactor F 420 . The Cofactor F 420 of each of the methanogens absorbs a first light produced by a light source thereby resulting in excitation and relaxation of the Cofactor F 420 of each of the methanogens. Excitation and relaxation of the Cofactor F 420 of each of the methanogens generates a second emission light from the Cofactor F 420 of each of the methanogens. Also described is a methanogen detection system and method.
1 . A methanogen detection device, comprising:
a sample inlet;
a sample transport channel in fluid communication with the sample inlet; and
a capillary flow channel in fluid communication with the sample transport channel, the capillary flow channel configured to support flow of a sample comprising methanogens and each of the methanogens comprising Cofactor F 420
wherein the Cofactor F 420 of each of the methanogens absorbs a first light produced by a light source thereby resulting in excitation and relaxation of the Cofactor F 420 of each of the methanogens, and
wherein excitation and relaxation of the Cofactor F 420 of each of the methanogens generates a second emission light from the Cofactor F 420 of each of the methanogens.
2 . The methanogen detection device of claim 1 , further comprising:
a capillary flow medium within the capillary flow channel, the capillary flow medium configured to support the sample thereon.
3 . The methanogen detection device of claim 1 , further comprising:
an optical filter downstream from the second emission light.
4 . The methanogen detection device of claim 1 , further comprising:
a transparent top layer, wherein the sample inlet extends through the transparent top layer;
a middle layer, wherein the middle layer defines the sample transport channel and the capillary flow channel; and
a transparent base layer supporting the sample transport channel and the capillary flow channel.
5 . The methanogen detection device of claim 4 , further comprising:
a sample well extending from the transparent top layer, wherein the sample well defines the sample inlet.
6 . The methanogen detection device of claim 1 , wherein the sample inlet is configured to receive the sample, and
wherein the sample received in the sample inlet flows from the sample inlet to the sample transport channel and from the sample transport channel to the capillary flow channel.
7 . The methanogen detection device of claim 3 , wherein the optical filter is configured to transmit a band of the second emission light comprising a wavelength range of 470 ± 2.5 nm.
8 . The methanogen detection device of claim 3 , wherein a light detector is configured to detect an intensity of the second emission light transmitted through the optical filter.
9 . The methanogen detection device of claim 1 , wherein the sample transport channel is serpentine.
10 . The methanogen detection device of claim 1 , wherein the capillary flow channel comprises a cylindrical chamber at a distal portion of the capillary flow channel.
11 . The methanogen detection device of claim 2 , wherein the capillary flow medium is chromatography paper.
12 . The methanogen detection device of claim 5 , wherein the sample well comprises a sealing cap.
13 . The methanogen detection device of claim 1 , further comprising:
a vent in fluid communication with at least one of the following: the sample inlet, the sample transport channel, the capillary flow channel, or any combination thereof.
14 . The methanogen detection device of claim 1 , wherein the sample further comprises a lysis solution configured to break down a cell wall of each of the methanogens.
15 . The methanogen detection device of claim 1 , further comprising:
at least one sample analysis channel in fluid communication with the sample transport channel, the at least one sample analysis channel separate from the capillary flow channel,
wherein the at least one sample analysis channel is configured to test for at least one of the following: pH, alkalinity, ammonia, filamentous bacteria, E. coli or any combination thereof.
16 . A methanogen detection system, comprising:
the methanogen detection device of claim 1 ;
a light source configured to produce the first light, the light source upstream to the capillary flow channel;
an optical filter downstream from the second emission light; and
a light detector downstream from the second emission light and the optical filter.
17 . The methanogen detection system of claim 16 , further comprising:
a capillary flow medium within the capillary flow channel, the capillary flow medium configured to support the sample thereon.
18 . The methanogen detection system of claim 16 , wherein the first light produced by the light source comprises a wavelength range of 420 ± 2.5 nm.
19 . The methanogen detection system of claim 16 , wherein the light detector comprises a camera, spectrophotometer, or photodiode.
20 . The methanogen detection system of claim 19 , wherein the light detector is the camera, and the camera comprises a smartphone camera.
21 . The methanogen detection system of claim 16 , further comprising:
a computing device in communication with the light detector, wherein the computing device is configured to generate a concentration of the methanogens in the sample based on the intensity of the second emission light detected by the light detector.
22 . A methanogen detection system, comprising:
a sample inlet;
a sample transport channel in fluid communication with the sample inlet;
a capillary flow channel in fluid communication with the sample transport channel, the capillary flow channel configured to support flow of a sample comprising methanogens and each of the methanogens comprising Cofactor F 420 ;
a light source configured to produce a first light, the light source upstream to the capillary flow channel,
wherein the Cofactor F 420 of each of the methanogens absorbs the first light produced by the light source thereby resulting in excitation and relaxation of the Cofactor F 420 of each of the methanogens, and
wherein excitation and relaxation of the Cofactor F 420 of each of the methanogens generates a second emission light from the Cofactor F 420 of each of the methanogens;
an optical filter downstream from the second emission light, wherein the optical filter is configured to transmit a band of the second emission light;
a light detector downstream from the second emission light and the optical filter, wherein the light detector is configured to detect an intensity of the second emission light transmitted through the optical filter; and
a computing device in communication with the light detector, wherein the computing device is configured to generate a concentration of the methanogens in the sample based on the intensity of the second emission light detected by the light detector.
23 . The methanogen detection system of claim 22 , further comprising:
a capillary flow medium within the capillary flow channel, the capillary flow medium configured to support the sample thereon.
24 . A methanogen detection method using the methanogen detection device of claim 1 , comprising:
providing a sample to the sample inlet, the sample comprising methanogens and each of the methanogens comprising Cofactor F 420 ;
irradiating the sample with a first light;
detecting an intensity of a second emission light emitted from the Cofactor F 420 ; and
generating a concentration of the methanogens in the sample based on the intensity of the second emission light.
25 . A methanogen detection method using the methanogen detection system of claim 16 , comprising:
providing a sample to the sample inlet, the sample comprising methanogens and each of the methanogens comprising Cofactor F 420 ;
irradiating the sample with the first light;
detecting an intensity of a second emission light emitted from the Cofactor F 420 ; and
generating a concentration of the methanogens in the sample based on the intensity of the second emission light.