Methods for treating plague
Provided herein are methods for using compositions that include a fusion protein having a YscF protein domain, a mature F1 protein domain, and a LcrV protein domain. In one embodiment the composition is used to confer immunity to plague, such as pneumonic plague, caused by Yersinia pestis. In one embodiment, the composition is administered to a mucosal surface, such as by an intranasal route. In one embodiment, the administration to a mucosal surface includes a vector that has a polynucleotide encoding a fusion protein, where the fusion protein includes a YscF protein domain, a mature F1 protein domain, and a LcrV protein domain. The administration is followed by a second administration by a different route, such as an intramuscular route. The second administration includes a fusion protein having the same three domains, and in one embodiment the fusion protein is the same one administered to a mucosal surface.
1. A method comprising:
administering a first composition to a subject by an intranasal route,
wherein the first composition comprises a vector comprising a polynucleotide encoding a fusion protein,
wherein the fusion protein comprises a YscF protein domain, a mature F1 protein domain, and a LcrV protein domain; and
administering a second composition to the subject by an intramuscular route,
wherein the second composition comprises the fusion protein, wherein the fusion protein is isolated, and
wherein the intramuscular administration is after the intranasal administration.
2. The method of claim 1 wherein the fusion protein comprises at least one linker, wherein the linker is present between two of the domains.
3. The method of claim 1 wherein the fusion protein comprises a His-tag.
4. The method of claim 1 wherein the vector is a replication defective adenovirus vector.
5. The method of claim 4 wherein the defective adenovirus vector is type-5 (Ad5).
6. The method of claim 1 wherein the fusion protein comprises the YscF protein, the mature F1 protein, and the LcrV protein.
7. The method of claim 1 wherein the intramuscular administration is at least 7 days after the intranasal administration.
8. The method of claim 1 wherein the subject is a human.
9. The method of claim 1 wherein the administering confers immunity to plague caused by Yersinia pestis.
10. The method of claim 9 wherein the plague is pneumonic plague or bubonic plague.
11. A method comprising:
administering a composition to a subject by an intranasal route,
wherein the composition comprises a vector comprising a polynucleotide encoding a fusion protein,
wherein the fusion protein comprises a YscF protein domain, a mature F1 protein domain, and a LcrV protein domain; and
administering a second administration of the composition to the subject by an intranasal route.
12. The method of claim 11 wherein the fusion protein comprises at least one linker, wherein the linker is present between two of the domains.
13. The method of claim 11 wherein the fusion protein comprises a His-tag.
14. The method of claim 11 wherein the vector is a replication defective adenovirus vector.
15. The method of claim 14 wherein the defective adenovirus vector is type-5 (Ad5).
16. The method of claim 11 wherein the fusion protein comprises the YscF protein, the mature F1 protein, and the LcrV protein.
17. The method of claim 11 wherein the subject is a human.
18. The method of claim 11 wherein the administering confers immunity to plague caused by Yersinia pestis.
19. The method of claim 18 wherein the plague is pneumonic plague or bubonic plague.