Detection of analytes using reorganization energy
The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.
1. A composition comprising an array of electrodes, each electrode comprising a covalently attached binding ligand and a covalently attached solvent accessible transition metal complex comprising a metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold.
2. A composition according to claim 1 wherein said solvent accessible transition metal complex has at least two coordination sites occupied by polar coordination groups.
3. A composition according to claim 1 wherein said solvent accessible transition metal complex has at least one coordination site occupied by a water molecule.
4. A composition according to claim 1 wherein said electrode further comprises a self-assembled monolayer.
5. A composition according to claim 1 wherein said solvent accessible transition metal complex is covalently attached to said electrode via a spacer.
6. A composition according to claim 5 wherein said spacer is a conductive oligomer.
7. A composition according to claim 1 wherein said solvent accessible transition metal complex is linked to said binding ligand to form a redox active complex.
8. A composition according to claim 1 wherein said binding ligand is covalently attached to said electrode via a conductive oligomer.
9. A composition according to claim 1 wherein said binding ligand will bind a protein.
10. A composition according to claim 1 wherein said binding ligand will bind a nucleic acid.
11. A composition according to claim 1 wherein said binding ligand is a protein.
12. A composition according to claim 1 wherein said solvent accessible transition metal complex is attached to said binding ligand.
13. A composition according to claim 6 wherein said conductive oligomer has the formula:
wherein Y is an aromatic group;
n is an integer from 1 to 50;
g is either 1 or zero;
e is an integer from zero to 10; and
m is zero or 1;
wherein when g is 1, B—D is a conjugated bond; and
wherein when g is zero, e is 1 and D is preferably carbonyl, or a heteroatom moiety, wherein the heteroatom is selected from the group consisting of oxygen, sulfur, nitrogen and phosphorus.
14. A composition according to claim 13 wherein said aromatic group is phenyl, g is 1, and B—D is an acetylene linkage.
15. A composition according to claim 6 wherein said conductive oligomer has the formula:
wherein
n is an integer from 1 to 50;
m is zero or 1;
C is carbon;
J is carbonyl or a heteroatom moiety, wherein the heteroatom is selected from the group consisting of oxygen, nitrogen, silicon, phosphorus and sulfur; and
G is bond selected from alkane, alkene or acetylene.
16. A composition according to claim 1 wherein said metal is gold.