Methods and compositions comprising
The invention relates to methods and compositions utilizing Renilla green fluorescent proteins (rGFP), and Ptilosarcus green fluorescent proteins (pGFP). In particular, the invention relates to the use of Renilla GFP or Ptilosarcus GFP proteins as reporters for cell assays, particularly intracellular assays, including methods of screening libraries using rGFP or pGFP.
1. A polynucleotide encoding a Renilla green fluorescent protein (GFP), wherein said polynucleotide comprises the nucleotide sequence of SEQ ID NO: 1.
2. The polynucleotide of claim 1 , further comprising a promoter operably linked to said polynucleotide.
3. The polynucleotide of claim 1 , further comprising a nucleic acid of interest.
4. The polynucleotide of claim 3 , wherein said nucleic acid of interest encodes a reporter protein or selectable marker.
5. The polynucleotide of claim 3 , wherein said nucleic acid of interest encodes a random peptide.
6. The polynucleotide of claim 3 , wherein said nucleic acid of interest is a cDNA.
7. The polynucleotide of claim 1 , wherein said Renilla GFP is a fusion protein.
8. The polynucleotide of claim 7 , wherein said Renilla GFP contains an insertion.
9. A vector comprising the polynucleotide of claim 1 .
10. The vector of claim 9 , wherein said vector is a retroviral vector.
11. The vector of claim 9 , further comprising an IRES sequence.
12. A cell comprising the vector of claim 9 .