Electrowetting electrode device with electromagnetic field for actuation of magnetic-bead biochemical detection system
A detecting device for biochemical detections is provided. The detecting device includes a first substrate, a magnetic layer located on the first substrate, an isolation layer located on the magnetic layer, at least a first electrode located on the isolation layer, a first dielectric layer located on the first electrode, a first hydrophobic layer located on the first dielectric layer, a second substrate, at least a second electrode located on the second substrate and having a cathode and an anode, a second dielectric layer located on the second electrode' and a second hydrophobic layer located on the second dielectric layer. The first electrode is zigzag-shaped, and the cathode and the anode of the second electrode are comb-shaped and interlaced with each other.
1. A magnetic beads biochemical detecting device for detecting a target in a plurality of magnetic beads of a droplet, comprising:
a first substrate;
a magnetic layer located on said first substrate;
an isolation layer located on said magnetic layer;
at least a first electrode located on said isolation layer, wherein said first electrode is zigzag-shaped;
a first dielectric layer located on said first electrode;
a first hydrophobic layer located on said first dielectric layer;
a second substrate;
at least a second electrode located on said second substrate and having a cathode and an anode, wherein said cathode and said anode are comb-shaped and are interlaced with each other;
a second dielectric layer located on said second electrode; and
a second hydrophobic layer located on said second dielectric layer;
wherein said first substrate and said second substrate are respectively configured to make said first electrode and said second electrode face to each other and to form a gap therebetween; said droplet is movable in said gap by a voltage difference produced between said first electrode and said second electrode; a magnetic force for attracting said plurality of magnetic beads is induced by applying currents only on said first electrode; and a positive-and-negative interlaced electric field is generated by applying currents on said cathode and said anode for initiating a certain reaction of said target to further generate a signal of current to be detected.
2. The detecting device according to claim 1 , wherein said magnetic layer is magnetized by said magnetic force.
3. The detecting device according to claim 1 , wherein a magnetic line of force of said magnetic force is closed by said magnetic layer so as to reduce a dissipation of said magnetic line of force and enhance said magnetic force.
4. The detecting device according to claim 1 , wherein said reaction is an oxidation-reduction reaction.
5. The detecting device according to claim 1 , wherein said first substrate is a silicon substrate.
6. The detecting device according to claim 1 , wherein said first electrode is made of a metal.
7. The detecting device according to claim 1 , wherein said magnetic layer is made of a permalloy.
8. The detecting device according to claim 1 , wherein said second substrate is a glass substrate.
9. The detecting device according to claim 1 , wherein said second electrode is made of a transparent and electrically conductive material.
10. The detecting device according to claim 1 , wherein said signal of current is forwarded by said cathode and said anode for being detected.